1 / 8

- +

EF reversal. + -. EF. - +. E. A. 360 min. 0 min. B. F. 420 min. 60 min. G. C. 540 min. 180 min. D. H. 660 min. 300 min. Zhang et al., Supplemental Fig. 1 Reversal of field polarity reverse d migration direction. + -. EF. Isolated hiPS cells.

amal
Download Presentation

- +

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. EF reversal +- EF -+ E A 360 min 0 min B F 420 min 60 min G C 540 min 180 min D H 660 min 300 min Zhang et al., Supplemental Fig. 1 Reversal of field polarity reversed migration direction.

  2. + - EF Isolated hiPS cells big colony of hiPS cells A E 0 min B F 90 min G C 180 min H D EF EF + - + - Zhang et al., Supplemental Fig. 2 Electrotaxis of an Isolated and a large colony of hiPS cells.

  3. * * * * * * Zhang et al., Supplemental Fig. 3 Displacement along X axis.

  4. A SSEA-4 B Oct-4 C merger Zhang et al. SupplementalFig. 4. Small electric fields did not alter expression of stem cell markers.

  5. C A NO EF + Y- 27632 NO EF B D EF EF+Y-27632 + - + - Zhang et al., supplemental Fig. 5 ROCK inhibition altered cell morphology and induce protrusions in hiPS cells.

  6. 100mV/mm NO EF EF + - A 4 1 3 2 4 1 2 3 6 5 5 0 h 0 h 1 4 2 3 4 3 6 2 1 5 3 h 5 3 h C B * * Zhang et al. Supplemental Fig. 6 EFs guide anodal migration of dermal fibroblasts in 2D.A, representative images showing cells migrate anodally when treated with EFs (right panel), as compared to non-EFs treated controls (left panel); B and C, quantitative analysis showing significantly increased directedness (B) and trajectory speed (C) of cells by EFs stimulation. *, P<0.01.

  7. 200mV/mm NO EF EF + - A 1 1 2 2 0 h 0 h 1 2 1 2 6 h 6 h C B * * Zhang et al. Supplemental Fig. 7 EFs guide anodal migration of dermal fibroblasts in 3D. A, representative images showing cells migrate anodally in 3D when treated with EFs (right panel), as compared to non-EFs treated controls (left panel); B and C, quantitative analysis showing significantly increased directedness (B) and trajectory speed (C) of cells in 3D by EFs stimulation. *, P<0.01.

  8. 50mV/mm 100mV/mm A 0 h 3 h 6 h B C * * * * Zhang et al. Supplemental Fig. 8 Galvanotaxis of hiPS cells in 3D at 50mV/mm and 100mV/mm. A, representative images showing anodal migration of hiPS cells at 50mV/mm (left panel) and 100 mV/mm (right panel); B and C, quantitative analysis showing the directedness (B) and migration speed (C) of hiPS cells at indicated EFs strength. *, P<0.01.

More Related