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Sunday, 20 June 2010. Theresa Aveling University of Pretoria South Africa. SEED HEALTH COMMITTEE. Theresa Aveling , South Africa. Sunday, 20 June 2010 . 2007-2010 Seed Health Committee. Sunday, 20 June 2010. PAST, PRESENT and FUTURE Rules development Introduction of new methods

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Seed health committee

Sunday, 20 June 2010

  • Theresa Aveling

  • University of Pretoria South Africa

SEED HEALTH COMMITTEE

Theresa Aveling, South Africa


2007 2010 seed health committee

Sunday, 20 June 2010

2007-2010 Seed Health Committee


Sunday, 20 June 2010

PAST, PRESENT and FUTURE

Rules development

Introduction of new methods

Introduction of Rules changes

Publications

Seed health handbook

Annotated list of seed-borne diseases

Workshops and symposia

Proficiency testing

Special projects


Sunday, 20 June 2010

PAST and PRESENT: Rules development

Introduction of new methods

2007: 7-021 Xanthomonas axonopodis pv. phaseoli and pv. phaseolis var. fuscans on Phaseolus vulgaris

2008: 7-022 Agar method for the detection of Michrodochium nivale on Tritichum spp.


Sunday, 20 June 2010

PAST and PRESENT: Rules development

Introduction of new methods

2008: 7-023 Detection of Pseudomonas savastanoi pv. phaseolicola on Phaseolus vulgaris

2008: 7-024 Detection of Pea Early Browning Virus and Pea Seed-borne Mosaic Virus on Pisum sativum


Sunday, 20 June 2010

PAST and PRESENT: Rules development

Introduction of new methods

2009: 7-025 Detection of Aphelenchoides besseyi on Oryza sativa

2010: 7-026 Detection of Squash Mosaic Virus, Cucumber Green Mottle Mosaic Virus and Melon Necrotic Spot Virus in cucurbits


Sunday, 20 June 2010

Future 2010-2013: Rules development

Introduction of new methods

Xanthomonas hortorum pv. carotae on Daucus carota – PCR method

Xanthomonas campestris pv. campestris – disinfected Brassica seeds

Fusarium spp. on Zea mays

Tobamo viruses/pepper and Lycopersicon esculentum – bioassay

Pyrenophora spp. on Hordeum vulgare


Sunday, 20 June 2010

Future 2010-2013: Rules development

Introduction of new methods

Ustilago nuda on Hordeum vulgare (Nordic)

Amalgamation of 3 methods for Linum

At SHC, it was decided to align the three methods 7-007, 7-017, 7-018 in a single method to detect Alternaria linicola, Botrytis cinerea, and Colletotrichum lini

Peer validation included 3  labs: Volcani (R Kohen), SNES (V Grimault) and Canadian Food Inspection Agency (S Brière)

The parameters which were different in the three ISTA methods were compared: incubation T°, light during incubation and length of incubation were selected as parameters


A pretest was carried out in one lab at SNES, to define the conditions to be tested for the peer validation.

As new ISTA methods include the addition of 50 to 130 mg/L of Streptomycin, we decided to add this parameter for comparison:

With/withoutantibiotics: Streptomycin 0; 50; 130 and 200 mg/L at 20°C dark

Dark, Light/dark, dark/NUV

Temperature: 20, 22°C

Lots: 15% per pathogen, 4*100 seeds

Results of pretest did not show significant difference between conditions


Sunday conditions to be tested for the peer validation., 10 June 2010

Test plan: Peer validation

3 levels of contamination per pathogen (healthy, low, medium)

4*100 seeds per condition

5 Conditions :

1: Malt agar without streptomycin, 20°C, in darkness as 7-007

2: Malt-agar with streptomycin 50 mg/L, 20°C, in darkness during 9 days and 12h NUV/12h dark.

3: Malt agar without streptomycin, 22°C, 12h NUV/12h dark as 7-0017 and 7-0018

4: PDA with streptomycin 50 mg/L, 20°C, in darkness during 9 days and NUV

5: PDA with streptomycin 50 mg/L, 20°C, 12h light, 12h dark

Evaluation 5, 7, 9 days


Sunday, 20 June 2010 conditions to be tested for the peer validation.

Results of peer validation:

Statisticalanalysis

All conditions testedallowdetection and identification of the 3 pathogens

We will propose a method based on these conditions for the update of the ISTA methods for detection of A. linicola, C. liniand B. cinerea:

50 ppm Streptomycin

20°C

Malt Agar or PDA

Dark 9 days and NUV 12h if needed for sporulation

Evaluation 5, 7, 9 days


Sunday, 20 June 2010 conditions to be tested for the peer validation.

Future 2010-2013: Rules development

Introduction of Rules changes

Editorial and update of taxonomical names

Consistancy


Sunday, 20 June 2010 conditions to be tested for the peer validation.

PAST, PRESENT and FUTURE: Publications

Seed health handbook

CHP 1 Introduction

CHP 2 Sampling

CHP 3 Design & interpretation of seed health tests

CHP 4 Basic techniques

CHP 5 Testing for fungi

CHP 6 Testing for bacteria

CHP 7 Testing for viruses

CHP 8 Testing for nematodes


Sunday, 20 June 2010 conditions to be tested for the peer validation.

PAST, PRESENT and FUTURE: Publications

Seed health handbook

CHP 9 Immunology

CHP 10 Molecular techniques

CHP 11 Quality assurance

CHP 12 Seed treatment

Glossary

Useful references

Appendices???


Sunday, 20 June 2010 conditions to be tested for the peer validation.

PAST, PRESENT and FUTURE: Publications

Annotated list of seed-borne diseases

Large long-term project

To start in 2010/2011

Concentrate on crop


Sunday, 20 June 2010 conditions to be tested for the peer validation.

PAST and PRESENT: Workshops and symposia

7-11 April 2008: Seed Health workshop in Pretoria, South Africa.21 participants from 11 countries: Brazil (1), Israel (1), Kenya

(1), Nigeria (1), Seychelles (1),

South Africa (10),

Spain (1), Sudan (1),

Tanzania (2), United

States (1), Zambia (1)


Sunday, 20 June 2010 conditions to be tested for the peer validation.

PAST and PRESENT: Workshops and symposia (cont.)

Instructors: R Blanco (University of Almeria, Spain), K Tylkowska (Agricultural University of Poznan, Poland), C Nieves Mortensen (University of Copenhagen, Denmark), T Goszczynska (Plant Protection Research Institute, South Africa), R Mumford (Central Science Laboratory, United Kingdom)and M Lesufi (Department of Agriculture, South Africa)


Sunday, 20 June 2010 conditions to be tested for the peer validation.

PAST and PRESENT: Workshops and symposia (cont.)

ISTA 6th SEED HEALTH SYMPOSIUMBerg en DalRestcamp, Kruger National Park, South Africa, 14-18 April, 2008


Sunday, 20 June 2010 conditions to be tested for the peer validation.

PAST and PRESENT: Workshops and symposia (cont.)

Organiser: T Aveling. 1st time in Africa. 65 participants from 28 countries. 22 paper and 14 poster presentations and 2 workshops


Sunday, 20 June 2010 conditions to be tested for the peer validation.

Organisers: V Grimault and V Cockerell

21 participants from 16 countries (Europe, Kenya, Israel, Brazil)

PAST and PRESENT: Workshops and symposia (cont.)

Detection of Botrytis cinerea on Linum seeds and on sunflower seeds; detection of Fusarium spp. on wheat seeds

GEVES-SNES Angers France. 2-5th March 2010


Sunday, 20 June 2010 conditions to be tested for the peer validation.

2 sessions, one on Fusarium and one on Botrytis

11 lectures dealing with epidemiology, taxonomy, detection methods

Lectures by scientists from INRA, University of Angers and by specialists from Canada, Scotland and France.


Sunday, 20 June 2010 conditions to be tested for the peer validation.

FUTURE: Workshops

and symposia

2011

Seed Health workshop

Aleppo, Syria

7th Seed Health Symposium

Aleppo, Syria

2012

Possible Workshop in Spain


Sunday, 20 June 2010 conditions to be tested for the peer validation.

PAST and PRESENT: Proficiency testing

7-003 Botrytis cinereaon Helianthus annus

7-013 Ustilagonudaon Hordeumvulgare

7-001a,b and 7-002a,b Alternariadauciand A. radicinaon Daucuscarota

7-020 Xanthomonashortorumpv. carotae on Daucuscarota

7-010, 7-011, 7-012 Dreschleraoryzae, Pyriculariaoryzaeand Alternariapadwickion Oryzae sativa

7-022 Microdochiumspp. on Triticum


Sunday, 20 June 2010 conditions to be tested for the peer validation.

FUTURE: Proficiency testing

7-009 Fusariumcircinatumon Pinustaedaand Pinuselliottii

7-006 Colletotrichumlindemuthianum on Phaseolusvulgaris

7-019 Xanthomonascampestrispv. campestris on Brassica spp.

7-013 Ustilagonudaon Hordeum

Depending on availability of contaminated seed ???


Sunday, 20 June 2010 conditions to be tested for the peer validation.

PAST, PRESENT and FUTURE: Special projects

Seed health testing sample size: Questionnaire

Phytosanitary aspects: Questionnaire

Seed treatment


Sunday, 10 June 2010 conditions to be tested for the peer validation.

Collaboration

Comparative tests for seed-borne fungal pathogens of spinach in co-operation with Lindsey du Toit of Washington State University

Standardization of Clavibactermichiganensis subsp. michiganensis test on tomato seeds in co-operation with EPPO


2010 2013 seed health committee

Sunday, 20 June 2010 conditions to be tested for the peer validation.

2010-2013 Seed Health Committee


Thank you

THANK YOU! conditions to be tested for the peer validation.


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