Step up year 1 practical laboratory session iron analysis
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Step-Up Year 1 Practical Laboratory Session: Iron Analysis PowerPoint PPT Presentation


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Step-Up Year 1 Practical Laboratory Session: Iron Analysis . Iron (Fe) . Iron is vital for a number of physiological processes Occurs naturally in food sources Also occurs naturally in our water . Iron in our water . Should we be concerned?

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Step-Up Year 1 Practical Laboratory Session: Iron Analysis

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Step up year 1 practical laboratory session iron analysis

Step-Up Year 1 Practical Laboratory Session: Iron Analysis


Iron fe

Iron (Fe)

Iron is vital for a number of physiological processes

  • Occurs naturally in food sources

  • Also occurs naturally in our water


Iron in our water

Iron in our water

  • Should we be concerned?

  • Iron in water is found in two forms:- Ferrous (Fe2+)- Ferric (Fe3+)

Fe3 + 3H2O  Fe(OH)3 + 3H+


Iron in our water1

Iron in our water

  • A range of solutions from no iron to high iron content

  • Ferrous iron in water has addedto it thiocyanate (SCN)

Fe(SCN)(H20)5]2+


Iron in our water2

Iron in our water

  • How do we decide what should be classed as ‘low’ or ‘high’ iron content?

  • Calibration Curve – our baseline to compare against


Iron analysis

Iron Analysis

  • Using a Spectrophotometer at a wavelength 490nm to measure Absorbance (nm)

  • Using the calibration curve, we can determine unknown Iron Concentrations


Before you start

Before you start

  • Read the protocol thoroughly

  • Always read the protocol before you start and follow H&S

  • Remember Beer-Lambert Law

?

  • AnyQuestions

  • Absorbance =ε.c.l

  • where ε = constant

  • c = concentration

  • l = path length through solution


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