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The Human Metabolome Project

The Human Metabolome Project. Lori Querengesser Genome Prairie Dept. of Computing Science, University of Alberta loriq@ualberta.ca www.metabolomics.ca. Human Metabolome Project. What is it? Who’s involved? What’s the HMDB and the HML? How can it help?. Human Metabolome Project.

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The Human Metabolome Project

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  1. The Human Metabolome Project Lori Querengesser Genome Prairie Dept. of Computing Science, University of Alberta loriq@ualberta.ca www.metabolomics.ca

  2. Human Metabolome Project • What is it? • Who’s involved? • What’s the HMDB and the HML? • How can it help?

  3. Human Metabolome Project • $7.5 million Genome Canada Project launched in Jan. 2005 • Mandate to quantify (normal and abnormal ranges) and identify all metabolites in urine, CSF, plasma and WBC’s • Make all data freely and electronically accessible (HMDB) • Make all cmpds publicly available (HML)

  4. Human Metabolome Project • Purpose is to facilitate Metabolomics • Objective is to improve • Disease identification, prognosis & monitoring • Drug metabolism and toxicology • Linkage between metabolome & genome • Development of software for metabolomics

  5. Why 1 mM? • Equals ~200 ng/mL • Limit of detection by NMR • Limit of facile isolation/separation by many analytical methods • Excludes environmental pollutants • Most IEM indicators and other disease indicators have concentrations >1 mM • Need to draw the line somewhere

  6. Human Metabolome Project • What is it? • Who’s involved? • What’s the HMDB and the HML? • How can it help?

  7. David Wishart Comp. Sci. U of Alberta Proj. Leader Brian Sykes Biochemistry U of Alberta NMR spect. Russ Greiner Comp. Sci. U of Alberta Bioinformatics Hans Vogel Biochemistry U of Calgary NMR spect. Fiona Bamforth Clin. Chemistry U of Alberta Sample Acq. Derrick Clive Chemistry U of Alberta Synthesis Liang Li Chemistry. U of Alberta MS/Separation Mike Ellison Biochemistry U of Alberta MS/Separation.

  8. Human Metabolome Project • What is it? • Who’s involved? • What’s the HMDB and the HML? • How can it help?

  9. HMDB is the public face of the HMP Freely web-accessible database providing detailed information on metabolites, chemistry, enzymes, diseases, pathways Links metabolome to genome HML is the Human Metabolome Library Repository of chemical samples for public redistribution Includes purchased, isolated & synthesized cmpds (many unique or rare cmpds) The HMDB & the HML

  10. Human Metabolome Database www.hmdb.ca

  11. The HMDB MetaboCard

  12. The Human Metabolome Library • 10 mg – 10 g of ~1400 human metabolites • may be purchased for use as references or standards by commercial or clinical testing labs • may be obtained free of charge for University and Hospital researchers interested in collaborative research or those who will acknowledge the use and source of the compounds in publications

  13. The HML Allows One To… • Access rare or unusual metabolites as references or standards for MS, HPLC, GC-MS or NMR analyses • Compare newly isolated compounds with known cmpds (saves on reinventing the wheel) • Use these compounds as precursors to synthesize new metabolites

  14. HML & HMDB Status • 825 compounds ID’d to date • 600 compounds with sources • 325 compounds purchased • 100 compounds fully characterized and archived in HML • 800 partial entries in the HMDB • Full set of 235 HMDB expected July 1 • Text based and will be continuously updated as we progress

  15. Metabolomics LIMS System

  16. Metabolomics LIMS System

  17. Metabolomics LIMS System

  18. Human Metabolome Project • What is it? • Who’s involved? • What’s the HMDB and the HML? • How can it help?

  19. We Need You! • Always looking for collaborations • We have ~$8 million in equipment (NMR, HPLC, FT-ICR, QTOFs, etc) ready and waiting for samples to be analyzed • We have 4 full-time programmers to develop software you may want or need • Existing collaborations on cachexia, prostate cancer, pneumonia, obesity

  20. Thanks to...

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