TCR Identification. February 13, 2007. The elusive TCR.
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February 13, 2007
“The T-cell receptor-the molecule in the T-cell membrane capable of specifically binding antigen-is the San Andreas Fault of immunology: here the plates rub against each other, shifting gradually and unnoticed, and a major “earthquake” may occur at any moment.”
“Few issues in science have proved as difficult to resolve and as subject to contention as the nature of the TCR.”
Jean Marx (writer for Science) 1983
“The study of the T cell receptor has been the subject of many studies, many of which have led to diverse and controversial findings. At the present time, the consensus is that the receptor is not conventional surface Ig as found on B cells.”
Unanue and Benacerraf 1984
Steve Hedrick, David Cohen, Ellen Nielsen, and Mark Davis
Laboratory of Immunology
Sequence of TM86 clone reveals an immunoglobulin like molecule
Blast search of TM86 identifies it to be V1, but cytochrome c responses are all V3!
LOCUS 1109237A 123 aa linear VRL 26-JUN-1996
DEFINITION T cell receptor VDJbeta 1.9.2.
VERSION 1109237A GI:224610
DBSOURCE prf: locus 1109237A;
KEYWORDS T Cell Receptor V beta Gene; Mouse; Seq Determination; 450bp;
96AAs; Use Limited Repertoire of Gene; Somatic Diversification at
V-D-J Region; DNA Blot; Single Gene Subfamily; Variability Plot;
Hydrophobicity; beta Sheet Potential.
SOURCE Murid herpesvirus 1 (Murine cytomegalovirus)
ORGANISM Murid herpesvirus 1
Viruses; dsDNA viruses, no RNA stage; Herpesviridae;
REFERENCE 1 (residues 1 to 123)
AUTHORS Barth,R.K., Kim,B.S., Lan,N.C., Hunkapiller,T., Sobieck,N.,
Winoto,A., Gershenfeld,H., Okada,C., Hansburg,D., Weissman,I.L. and
TITLE The murine T-cell receptor uses a limited repertoire of expressed V
beta gene segments
JOURNAL Nature 316 (6028), 517-523 (1985)
/organism="Murid herpesvirus 1"
/note="Immunoglobulin domain variable region (v)
subfamily; members of the IGv subfamily are components of
immunoglobulins and T-cell receptors; cd00099"
1 vslclvetal mntkitqspr ylilgranks leceqhlghn amywykqsae kppelmflyn
61 lkqlirnetv psrfipecpd ssklllhisa vdpedsavyf cassqtggnt evffgkgtrl
Complete primary structure of a heterodimeric T-cell receptor deduced from cDNA sequences.
* Saito H,
* Kranz DM,
* Takagaki Y,
* Hayday AC,
* Eisen HN,
* Tonegawa S.
Two related, but distinct, cDNA clones have been isolated and sequenced from a functional murine cytotoxic T-lymphocyte clone. The genes corresponding to these cDNA are expressed and rearranged specifically in T cells and both have similarities to immunoglobulin variable and constant region genes. It is concluded that these genes code for the two subunits of the heterodimeric antigen receptor on the surface of the T cell; its complete deduced primary structure is presented.
PMID: 6330561 [PubMed - indexed for MEDLINE]
Nature. 1984 Jun 28-Jul 4;309(5971):757-62.
Transfection of these two receptors does not recapitulate the specificity of the 2C TCR!
John Kappler, Ralph Kubo, Kathryn Haskins, Charles Hannum, Michele Pigeon, and Pippa Marrack
National Jewish Hospital, Denver
Brad McIntyre and Jim Allison
UT, Smithville-> Berkeley->MSK
Salk Institute, La Jolla
“REX line was obtained from a patient with T cell lymphoblastic lymphoma which gave rise to spontaneously growing subclones as isolated by limiting dilution technique.”