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Acquire ovarian cancer cell lines from various repository

Supplementary Figure 1. A. Acquire ovarian cancer cell lines from various repository ATCC (6), CRT (2), DSMZ (2), ECACC (7), Kyoto U (24), RIKEN (1). Establish EM Phenotypic Characterisation. Validate EMT Marker Expression. Cell line expansion & banking SGOCL (43). Create EMT Spectrum.

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Acquire ovarian cancer cell lines from various repository

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  1. Supplementary Figure 1 A Acquire ovarian cancer cell lines from various repository ATCC (6), CRT (2), DSMZ (2), ECACC (7), Kyoto U (24), RIKEN (1) Establish EM Phenotypic Characterisation Validate EMT Marker Expression Cell line expansion & banking SGOCL (43) Create EMT Spectrum Immunofluorescence Staining DNA, RNA, Protein Extraction Functional Correlation Downstream Analysis B RNA Cell QA Agilent Bioanalyzer Functional Assays Affymetrix GeneChip Human Gene 1.0 ST Array Migration Assay Invasion Assay Anoikis Assay Colony Assay EMT RT2 Profiler qPCR Array Parametric / Nonparametric Analysis Endpoint Readout Image Analysis Gene Expression Profiling Phenotype-specific Gene Signatures Phenotype-specific Gene Expression

  2. Supplementary Figure 2

  3. Supplementary Figure 3 Migration B Invasion A Epithelial Caov3 PEO1 OVCAR-3 OVCAR8 Caov3 PEO1 OVCAR3 OVCAR8 Intermediate E IGROV1 OVCA433 OVCA429 PEO4 IGROV1 OVCA433 OVCA429 Intermediate M CH1 DOV13 SKOV3 Hey C2 CH1 DOV13 SKOV3 Hey C2 Mesenchymal A1847 A2780 Hey A8 TykNu A1847 A2780 Hey A8 TykNu E Invasive Front F D C OVCA433 HeyC2 SKOV3 subcutaneous tumour. Measurements in centimeters (cm) SKOV3 subcutaneous tumours Day 59

  4. Supplementary Figure 4 Control 100 nM 5 mM 500 nM OVCAR3 (E) AZD0530 OVCA433 (Int E) AZD0530 SKOV3 (Int M) AZD0530 OVCAR10 (M) AZD0530

  5. Supplementary Figure 5 B A

  6. Supplementary Figure 6 A B

  7. Supplementary Figure 7 A 3-yr PFS 4-yr PFS 1-yr PFS 2-yr PFS B C

  8. Supplementary Figure 8 E-cad/DAPI N-Cad/DAPI

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