Analysis of protectin jeff messer guillermo vargas andres murillo
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Analysis of Protectin Jeff Messer Guillermo Vargas Andres Murillo. Like Most Things in Biology, Protectin Is Known by Several Names. 1F-5Ag H19 HRF20 MACIF MIRL P-18 CD59. The Basics at the DNA Level. Genomic location: 33845931 - 33859721 bp (33.8 Mb) on chromosome 11p13

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Analysis of protectin jeff messer guillermo vargas andres murillo

Analysis of ProtectinJeff MesserGuillermo VargasAndres Murillo


Like most things in biology protectin is known by several names
Like Most Things in Biology, Protectin Is Known by Several Names.

  • 1F-5Ag

  • H19

  • HRF20

  • MACIF

  • MIRL

  • P-18

  • CD59


The basics at the dna level
The Basics at the DNA Level Names

  • Genomic location: 33845931 - 33859721 bp (33.8 Mb) on chromosome 11p13

  • Total length: 1858 bp

  • Number of Exons: 3

  • View chromosome

  • View exons


Primary structure
Primary Structure Names

  • Sequence length: 128 amino acids

  • Functional part: 27 - 70



Family matters
Family Matters Names

  • Protectin is a member of the Ly-6 superfamily which are cell-surface molecules. Surprisingly, they both also share a very similar structure with snake venom neurotoxins.


Finally protectin s function
Finally…Protectin’s Function Names

  • This is a widely distributed protein that inhibits the membrane attack complex (MAC).

  • In other words, it protects cells from the immune system.



Function
Function Names


Cd59 deficiency
CD59 Deficiency Names

  • (1992) single nucleotide deletions in codon 16 (GCC to GC) and codon 96 (GCA to CA). Deletion in codon 16 resulted in a frameshift and introduced a stop codon at position 54

  • Hemolytic Anemia and Thrombosis

  • Causing Cerebral Infarction


More cd59 deficiencies
More CD59 Deficiencies Names

  • Paroxysmal Nocturnal Nemoglobinuria (Rosse, 1993), which is a disorder ofPIGA

  • Intravascular Hemolysis

  • Resulting in Anemia and later Leukemia


Why should i care
Why Should I Care? Names

  • Protectin may prolong the survival of animal organs in human transplantation.


Tertiary structure
Tertiary Structure Names

  • The tertiary structure was extrapolated through 2D NMR.

  • Relatively flat, disk shaped.

  • Main structure:

    • 2 anti-parallel beta-sheets in one “finger.”

    • 3 anti-parallel beta-sheets form the core.

  • 5 disulfide-bonded cysteine pairs.


3d structures on the web
3D Structures on the Web Names

  • CD59 – Human Complement Regulatory Protein, PDB ID: 1ERG

  • SCOP: Protein: CD59 from Homo sapiens

  • SCOP: Protein: Erabutoxin B (also neurotoxin B) from Sea snake (Laticauda semifasciata)


Protein structural evolution
Protein/Structural Evolution Names

  • Similar structure of snake venom neurotoxins




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