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“KEMRI Polio Lab Report 7 th Inter-Country meeting of Directors of poliovirus laboratories in the WHO-EMRO,The Hashemite Republic Of Jordan August 24-26, 2003 Kenya Medical Research Institute 24 years of Excellence in Health Research KEMRI Polio LAB.

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“KEMRI Polio Lab Report

7th Inter-Country meeting of Directors of poliovirus laboratories in the WHO-EMRO,The Hashemite Republic Of Jordan

August 24-26, 2003


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Kenya Medical Research Institute

24 years of Excellence in Health Research


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KEMRI Polio LAB.

  • Part of the Global Polio Lab. Network since 1995

  • Designated National/Inter-Country Lab. Serving Kenya, southern Sudan, Somalia, Djibouti, Eritrea , the Seychelles and St. Hellena islands (THE HORN OF AFRICAN REGION).

  • Support KEPI in Vaccine Potency Testing For (Measles, YF, Polio) and field surveillance

  • Supported by KEMRI, WHO (HQs, AFRO & EMRO), JICA and CDC


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EPI Lab. Management Structure

Lab. Director

Measles Lab. Manager

Polio Lab. Manager

Lab. Technical Supervisor

Measles Lab

Tissue Culture

Polio Lab.

Technologists , Engineer & Technicians


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STAFF DISPOSITION

  • CURRENT ACTIVE STAFF [100%] IN POLIO LAB

    - One Research Officer ( Lab. Director) Dr. P. Borus

  • One Research Scientist (Manager & Safety Officer) New Staff

  • Two Lab. Technologists Mr. P. Kingori

    Mr. M.Nth’iwa

    -One Senior Technician Mr Muchiri

    -Two Junior Technicians Ms J. Hassan and Ms E. Njeri

    • An Institute Engineer Mr. Okondo

    • Two Laboratory Assistants Mr. B. Mwangi and Ms.J. Lewett


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Internal Quality Control

  • Cell Sensitivity testing

  • Receive our cells (RD & L20B) with low passages from NICD in South Africa Done Quarterly.

  • Received NIBSC Polio Virus Reference Sabin strains.

  • Proficiency Testing

    - WHO-AFRO 100% (July 2003)

  • External Assessment by CDC & WHO ( 2002)

  • Regular Monitoring of Techniques

  • Standard Operating Procedures (SOPs) 2003 and WHO Manual used


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Internal QC (3)

  • Equipment

  • Calibration of equipment done by Kenya Bureau of Standards

  • Regular maintenance/servicing of equipment done by the Institute Engineer


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Networking

  • Meetings with KEPI on a weekly basis to reconcile data and to discuss surveillance issues

  • Relationship with WHO (WR, AFRO ,EMRO& Hqs) good. WHO Provide us With the Lab.:

  • Supplies

  • Assistance on data management

  • Internet connection and air conditioner

  • Useful exchange of ideas during AFRO meetings , during accreditation or external assessment

  • East Africa bloc Bulletins


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Important Operational Practices

  • Monthly In-house meetings

  • In-House computer Training in place.

  • Weekly meetings with KEPI

  • Recruited an Institute Engineer


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LABORATORY CONTAINMENT OF WILD POLIO VIRUS

  • MANY TYPES OF CONTAINERS USED.

  • WE HAVE REQUESTED FOR STANDARD STOOL CONTAINERS FROM GENEVA.

  • STOOLS STORED IN ORIGINAL CONTAINERS.

  • WATER PROOF TAPE STRAPPED AROUND THE CAP OF THE CONTAINER.

  • ALL CONTAINERS PACKED IN ONE AUTOCLAVE BAG FOR EACH MONTH AND DISCARDED AFTER ONE YEAR.


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Results- 1999

Country Sample Case P1 P2 P3

Somalia 82 41 4S 0 2Wild

Sudan 65 33 2S 0 2Wild

Djibouti 2 1 0 0 0

Total 149 75 6S 0 4Wild

KENYA 490 245 14S 0 1S



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Results 2000

Country sample Cases P1 P2 P3

Somalia 313 157 17S (73W) 26S 15S (6W)

Sudan 116 58 4S 1S 0

Djibouti 4 2 0 0 0

TOTAL 433 217 21S (73W) 27S, 15S(6W)

KENYA 609 304 24S 10S 29S


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Surveillance Indicators 2000

KENYA 53.5% 80.6% 99.2% 9.6%


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Results 2001

Country sample Cases P1 P2 P3 NPENT %

Somalia 261 131 10 12 20(13W)36 13.8

Sudan 173 86 5(1W) 0 0 24 13,9

Djibouti 2 1 0 0 0 0 0

Eritrea 34 17 0 0 0 5 7

Total 470 235 15(1W)13 20 (13W) 65 11.7

KENYA 542 271 8 9 2 38 7.0



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Results 2002

Country sample Cases P1 P2 P3 NPENT %

Somalia 216 108 9 18 14(6W) 23 10.6

Sudan 175 88 0 0 0 29 16.5

Eritrea 72 36 0 0 0 8 11.0

Total 463 232 9 18 14(6W) 60 12.9

KENYA 506 256 2 4 3 32 6.3






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PLAN OF ACTION- 2003

  • Strengthen regular contacts between the Lab. With KEPI.

  • To bring representatives from WHO-EMRO (Somalia , Djibouti, Sudan) counterparts on board during our weekly meetings (Present once a Month).

  • To identify contact persons in St. Hellena and Seychelles Islands.

  • Training of 2 staff in NICD South Africa .


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Recommendations

  • The number of countries supported and samples processed by the laboratory for EMRO has increased over the years- EMRO to consider more support to the lab in terms of :

  • Increasing the amount of money to USD 10,000

  • ASSIST LAB. WITH TRANSPORT-VEHICLE.

  • Training (Cell Culture /attachment of staff in their labs).

  • Invitations to EMRO meetings encouraged.


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ACKNOWLEDGEMENTS

-KEMRI,KEPI,JICA, WHO (HQs, AFRO & EMRO), CDC FOR SUPPORT


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