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Science 290: 2270-2271.

Science 290: 2270-2271. Science 290: 2270-2271. Localization of GFP-PSD-95 to dendritic spines. Nature Neuroscience 2, 804 - 811 (1999). Nat. Rev 3:791, 2002. Expression of PSD95 enhances synaptic clustering of AMPA but not NMDA receptors.

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Science 290: 2270-2271.

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  1. Science 290: 2270-2271.

  2. Science 290: 2270-2271.

  3. Localization of GFP-PSD-95 to dendritic spines. Nature Neuroscience 2, 804 - 811 (1999)

  4. Nat. Rev 3:791, 2002

  5. Expression of PSD95 enhances synaptic clustering of AMPA but not NMDA receptors

  6. Over-expression of PSD95 increases the amplitude and frequency of AMPA receptor mEPSC

  7. Recovery of NMDA receptor mediated EPSCs following MK801 block

  8. Common element in three structurally related proteins: PSD95/SAP90 (Post-synaptic density protein 95/synapse associated protein 90) Dlg (Drosophila tumor suppressor gene discs-large) Zo-1 (Zona occludens-1)

  9. PDZ domains Carboxylate binding loop ~90 aa 6 b sheets and 2 a helices Peptide binding in groove between aB and bB COOH binding loop between bA and bB {Gly-Leu-Gly-Phe (orginal name was GLGF repeats} N and C-terminus together on back of domain aA A aB bB F C Peptide recognition sequence consist of 4 C-terminal aa motif Forms hydrogen bonds and vander waals interactions with aa Very selective interaction D E N Binding groove C

  10. science.vol275/issue5296

  11. PDZ domain binding motifs science.vol275/issue5296

  12. MAGUK family of proteins (Membrane Associated Guanylate Kinases) N C GK SH2 PDZx

  13. S-SCAM 4 5 6 2 3 1 WW WW GRIP/ABP 4 5 6 7 2 3 1 PDZ SH3 GK PSD-95/SAP90 Chapsyn110 2 3 1 H ZO-1 Acid 2 3 1 CASK/Lin2 CB CaMK Mint1/Lin-10 2 1 PTB PICK1 CC

  14. Nature Neuroscience 3, 747 - 749 (2000)

  15. Post-synaptic expression of PSD95 enhances presynaptic development

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