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Science 290: 2270-2271. Science 290: 2270-2271. Localization of GFP-PSD-95 to dendritic spines. Nature Neuroscience 2, 804 - 811 (1999). Nat. Rev 3:791, 2002. Expression of PSD95 enhances synaptic clustering of AMPA but not NMDA receptors.

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Science 290 2270 2271

Science 290: 2270-2271.


Science 290 2270 2271

Science 290: 2270-2271.


Science 290 2270 2271

Localization of GFP-PSD-95 to dendritic spines.

Nature Neuroscience 2, 804 - 811 (1999)


Science 290 2270 2271

Nat. Rev 3:791, 2002


Science 290 2270 2271

Expression of PSD95 enhances synaptic clustering of AMPA but not NMDA receptors


Science 290 2270 2271

Over-expression of PSD95 increases the amplitude and frequency of AMPA receptor mEPSC


Science 290 2270 2271

Recovery of NMDA receptor mediated EPSCs following MK801 block


Science 290 2270 2271

Common element in three structurally related proteins:

PSD95/SAP90 (Post-synaptic density protein 95/synapse associated protein 90)

Dlg (Drosophila tumor suppressor gene discs-large)

Zo-1 (Zona occludens-1)


Science 290 2270 2271

PDZ domains

Carboxylate binding loop

~90 aa

6 b sheets and 2 a helices

Peptide binding in groove between aB and bB

COOH binding loop between bA and bB

{Gly-Leu-Gly-Phe (orginal name was GLGF repeats}

N and C-terminus together on back of domain

aA

A

aB

bB

F

C

Peptide recognition sequence consist of

4 C-terminal aa motif

Forms hydrogen bonds and vander waals

interactions with aa

Very selective interaction

D

E

N

Binding groove

C


Science 290 2270 2271

science.vol275/issue5296


Science 290 2270 2271

PDZ domain binding motifs

science.vol275/issue5296


Science 290 2270 2271

MAGUK family of proteins

(Membrane Associated Guanylate Kinases)

N

C

GK

SH2

PDZx


Science 290 2270 2271

S-SCAM

4

5

6

2

3

1

WW

WW

GRIP/ABP

4

5

6

7

2

3

1

PDZ

SH3

GK

PSD-95/SAP90

Chapsyn110

2

3

1

H

ZO-1

Acid

2

3

1

CASK/Lin2

CB

CaMK

Mint1/Lin-10

2

1

PTB

PICK1

CC


Science 290 2270 2271

Nature Neuroscience 3, 747 - 749 (2000)


Science 290 2270 2271

Post-synaptic expression of PSD95 enhances presynaptic development


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