Science 290: 2270-2271.
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Science 290: 2270-2271. Science 290: 2270-2271. Localization of GFP-PSD-95 to dendritic spines. Nature Neuroscience 2, 804 - 811 (1999). Nat. Rev 3:791, 2002. Expression of PSD95 enhances synaptic clustering of AMPA but not NMDA receptors.

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Science 290: 2270-2271.


Science 290: 2270-2271.


Localization of GFP-PSD-95 to dendritic spines.

Nature Neuroscience 2, 804 - 811 (1999)


Nat. Rev 3:791, 2002


Expression of PSD95 enhances synaptic clustering of AMPA but not NMDA receptors


Over-expression of PSD95 increases the amplitude and frequency of AMPA receptor mEPSC


Recovery of NMDA receptor mediated EPSCs following MK801 block


Common element in three structurally related proteins:

PSD95/SAP90 (Post-synaptic density protein 95/synapse associated protein 90)

Dlg (Drosophila tumor suppressor gene discs-large)

Zo-1 (Zona occludens-1)


PDZ domains

Carboxylate binding loop

~90 aa

6 b sheets and 2 a helices

Peptide binding in groove between aB and bB

COOH binding loop between bA and bB

{Gly-Leu-Gly-Phe (orginal name was GLGF repeats}

N and C-terminus together on back of domain

aA

A

aB

bB

F

C

Peptide recognition sequence consist of

4 C-terminal aa motif

Forms hydrogen bonds and vander waals

interactions with aa

Very selective interaction

D

E

N

Binding groove

C


science.vol275/issue5296


PDZ domain binding motifs

science.vol275/issue5296


MAGUK family of proteins

(Membrane Associated Guanylate Kinases)

N

C

GK

SH2

PDZx


S-SCAM

4

5

6

2

3

1

WW

WW

GRIP/ABP

4

5

6

7

2

3

1

PDZ

SH3

GK

PSD-95/SAP90

Chapsyn110

2

3

1

H

ZO-1

Acid

2

3

1

CASK/Lin2

CB

CaMK

Mint1/Lin-10

2

1

PTB

PICK1

CC


Nature Neuroscience 3, 747 - 749 (2000)


Post-synaptic expression of PSD95 enhances presynaptic development


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