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Control 7 day Stretch 7 day Prazosin. A. B. C. D. E. F. Supplementary Figure 1:

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Control 7 day Stretch 7 day Prazosin

A

B

C

D

E

F

Supplementary Figure 1:

Immunostaining of HIF-1a (A-C) and HIF-2a (D-F) in control (A,D), 7 day stretch (B,E) or 7 day prazosin (C,F) -treated muscles. Panels A-C were counter-stained with DAPI to visualize nuclei (blue) and panels D-F were counterstained with lectin to visualize capillaries (red). Representative images of each condition were obtained at a magnification of 400x. Arrows point to positive myonuclear staining for HIF-a proteins. Arrowhead in Panel B points to signal within the pericapillary area.


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