Igg detection using anti igg conjugated microparticles
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IgG Detection Using Anti- IgG Conjugated Microparticles. By Sarah Leung and Anbar Najam. Methods. Conjugated anti-IgG antibodies to 0.92 um microparticles 33% microparticle surface coverage with IgG 0.02% microparticle concentration in solution. Methods.

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IgG Detection Using Anti- IgG Conjugated Microparticles

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Igg detection using anti igg conjugated microparticles

IgG Detection Using Anti-IgG Conjugated Microparticles

By Sarah Leung and

AnbarNajam


Methods

Methods

  • Conjugated anti-IgG antibodies to 0.92 um microparticles

  • 33% microparticle surface coverage with IgG

  • 0.02% microparticle concentration in solution


Methods1

Methods

  • Positioned a 180 degree optical reflection probe directly above samples for detection on a two-well slide


Results

Results

  • 5*10-11 g/mL to 5*10-6 g/mLIgG serial dilutions


Results1

Results

  • 5*10-15 g/mL to 5*10-6 g/mL IgG serial dilutions


Discussion

Discussion

  • Results lacked evidence of any trends between antigen concentration and light scattering intensity

  • Possible explanations:

    • Coagulation of microparticles

    • High scattering due to 180 degree detection

    • Conjugation of antibody, rather than antigen, to microparticles


Next experiment

Next Experiment

  • Added TWEEN 80 to existing 0.92 um microparticle solution and evaluate 180 degree vs. 45 degree light scattering detection

Vs.

180 degrees

45 degrees


Results2

Results

5*10-14 g/mL to 5*10-6 g/mL IgG serial dilutions


Results3

Results

5*10-14 g/mL to 5*10-6 g/mL IgG serial dilutions


Discussion1

Discussion

  • 180 degree results lacked evidence of any trends between antigen concentration and light scattering intensity

  • With 45 degree probe, we found a detection limit of 10-6 g/ml


Igg detection using anti igg conjugated microparticles

Contact Angle Measurements of Anti-E. Coli and Anti-E. Coli Conjugated Microparticles on Superhydrophobic Surface


Methods2

Methods

  • Conjugated anti-E. coli antibodies to 0.92 um microparticles

  • 33% microparticle surface coverage with anti-E. coli

  • 0.02% microparticle concentration in solution


Methods3

Methods

  • Dispensed 10 ul droplet on to superhydrophobic surface.

  • Measured contact angle over 10 minutes

  • Droplets:

    • Deionized Water

    • Anti-E. coli -Conc. 5*10-7

    • Anti-E. coli conjugated to microparticles


Results4

Results

Anti-E. coli -Conc. 5*10-7


Discussion2

Discussion

  • Results oscillate and do not form a smooth curve.

  • Possible explanations:

    • Surface contamination

    • Droplet movement, vibration.

    • Position of droplet.

    • Size of droplet


Next experiment1

Next Experiment

Decrease the volume of the droplet from 10 ul to 3 ul for a more spherical shape.


Methods4

Methods


Results5

Results

Deionized Water


Future work

Future Work

Minimize the oscillations.

Change the position of the surface.

Determine an efficient way to dispense 3ul droplet on to superhydrophobic surface


Acknowledgement s

Acknowledgements

  • UA/NASA Space Grant

  • Dr. Jeong-Yeol Yoon

  • Lab group: Tremaine B. Powell, Jin-Hee Han, Brian C. Heinze, Phat L. Tran, Jennine N. Chesler, Dr. Keesung Kim, and Dr. Song


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