Isolation Technique. In nature microbial cultures are mixed Identification relies upon isolating individual colonies Testing requires pure cultures As a result isolation technique provides an essential microbiological tool. Mixed Culture from Raw Poultry. Streak Plate Isolation Principle.
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Quadrant 2 – Rotate the plate, enter the previous streak mark one or two times and then streak the upper portion of the second quarter of the plate with broad strokes.
Quadrant 3 – Rotate the plate, enter quadrant 2 one or two times and then streak with shorter more separated strokes from the top of the quadrant to the center.