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Supplemental Figure 3. G 2 -M. G 1. S. Counts. DNA content. Merge. PAR. TRF1.

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  1. Supplemental Figure 3 G2-M G1 S Counts DNA content Merge PAR TRF1 Figure Legend: BJ-EHLT fibroblasts were synchronized at the G1-S boundary with low dose of hydroxiurea (HU 0.5 mM for 16 hrs) and then released in fresh medium in the presence of 0.5 M RHPS4. Cells were fixed at the end of HU synchronization (G1) and after 6 (S-phase) and 10 hrs (G2-M phase). IF against PAR (green) and TRF1 (red) was performed. Upper panel: Flow cytometric analysis of DNA content; Lower panel: Representative images of IF acquired with a Leica Deconvolution microscope (magnification 63x) are reported.

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