1 / 1

Δ arrF

WT. ∆ arrF. WT. Δ arrF. Supplemental Figure 2. Confirmation of complete knockout of arrF gene in ∆ arrF mutant using real-time RT-PCR. Figure shows a curve representing the cycle-dependent fluorescence associated with amplification of arrF gene

aderes
Download Presentation

Δ arrF

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. WT ∆arrF WT ΔarrF Supplemental Figure 2. Confirmation of complete knockout of arrF gene in ∆arrF mutant using real-time RT-PCR. Figure shows a curve representing the cycle-dependent fluorescence associated with amplification of arrF gene product from RNA samples isolated from wild type and ∆arrF mutant. A primers-specific DNA was amplified in the sample of wild-type strain with the cross point (CP) value of ca. 17, whereas the DNA amplification was not observed in the ∆arrF mutant even after 40 cycles of amplification (inset). In the same conditions, the expression of internal control omlA was little affected by arrF mutation. These results strongly indicate that the arrF gene is completely deleted in the mutant and that, unlike Pseudomonas spp, A. vinelandii does not have other copy that is nearly identical to the arrF.

More Related