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Homo sapiens 1 MSSMWSEYTIGGVKIYFP--YKAYPSQLAMMNSILRGLNSKQ--------HCLLESPTGSGK 52

Fig. S1A. A. Q. I. Homo sapiens 1 MSSMWSEYTIGGVKIYFP--YKAYPSQLAMMNSILRGLNSKQ--------HCLLESPTGSGK 52 Pan troglodytes 1 MSSMWSEYTIGGVKIYFP--YKAYPSQLAMMNSILRGLNSKQ--------HCLLESPTGSGK 52 Equus caballus 1 SSAWSEYTIGGVKINFP--YKAYPSQLAMMNSIVRGLNSKQ--------HCLLESPTGSGK 51

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Homo sapiens 1 MSSMWSEYTIGGVKIYFP--YKAYPSQLAMMNSILRGLNSKQ--------HCLLESPTGSGK 52

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  1. Fig. S1A A Q I Homo sapiens 1 MSSMWSEYTIGGVKIYFP--YKAYPSQLAMMNSILRGLNSKQ--------HCLLESPTGSGK 52 Pan troglodytes 1 MSSMWSEYTIGGVKIYFP--YKAYPSQLAMMNSILRGLNSKQ--------HCLLESPTGSGK 52 Equus caballus 1 SSAWSEYTIGGVKINFP--YKAYPSQLAMMNSIVRGLNSKQ--------HCLLESPTGSGK 51 Bos taurus 1 MPKITLKGVTVDFP--FQPYKCQEEYMSKVLECLQEKV--------NGILESPTGTGK 49 Canis familiaris 1 MSSLWSEYTIGGVKITFP--YKAYPSQLAMMNSIVRGLNSKQ--------HCLLESPTGSGK 52 Mus musculus 1 MSSVLSDYTIGGVKIHFP--CRAYPAQLAMMNSIVRGLNSSQ--------HCLLESPTGSGK 52 Rattus norvegicus 1 MSSVLSEYTIGGVKIHFP--CRAYPAQLAMMNSIVRGLNSSQ--------HCLLESPTGSGK 52 Monodelphis 1 MSSTLSEYIIGGVKIHFP--CKAYPSQLALMNSIVRGLNSKQ--------HCLLESPTGSGK 52 Gallus gallus 1 MSSDVSQYTIGGVKIMFP--CKAYPSQLAMMNAIVKGLNNRQ--------HCLLESPTGSGK 52 Danio rerio 1 MAATPVEYTIGGVKINFP--CKAYPSQLAMMNSIIRGLNNGQ--------HCLLESPTGSGK 52 Xenopus laevis 1 MSSVLSEYTIGGVKILFP--CRAYPSQLAMMNSIMRGLNCKQ--------HCLLESPTGSGK 52 Trichoplax adhaerens 12IDNNRSYTISGVQVEFP--YKAYPSQLSMMSKIIQGLQQRK--------HCLLESPTGSGK 62 Ciona intestinalis 2 EEKPHVELMIGGVKIHFP--FKPYPSQLSMMSMIVKGLQRSE--------HCLLESPTGSGK 54 Oryza sativa 19 APGGRVYQVGGVPVEFP--YKPYGTQLAFMGRVIATLDRAR---RQGRSHALLESPTGTGK 74 Arabidopsis thaliana 20KNPKNVYQIGGLQVEFP--YQPYGTQLAFMSRVISTLDRAQ---RDGHCHALLESPTGTGK 76 Physcomitrella patens 2SLPHPSLRICGIPVDFP—-YKPYGTQLAFMGKVLAALEQSRHSHRSCSVNALLESPTGSGK 60 Vitis vinifera 7 TNPKNVYHIGGIAVEFP--YQPYGSQLAFMGRVISTLDRAQ---RDGHCHALLESPTGTGK 63 Ostreococcus tauri 26 GSTWRRMINGVEIRFP--LVPYASQIAVMSQVIRAVTSKQ--------CALVESPTGSGK 83 Bombyx mori 58 QNEITIMIAGVKVNLP--VNPYGTQKALIHQVIKTIKAAQ--------NCLLESPTGSGK 108 Apis mellifera 1 MPDVTINNIIINFP--FKPYSIQEEYMAKVIQCLQNSK--------NGVLESPTGTGK 48 Drosophila 1 MPESLIAGIPVHFP--FEPYPVQRAYMEKVIQCLRDGT--------NGVLESPTGTGK 48 Caenorhabditis elegans 72EQLMMLGVPVRVPRGLSLYSTQKLMIVRILTALKNSQ--------NVLGESPTGSGK 121 Aedes aegypti 1 MPEYQINGITVNFP--FEPYQVQRDYMSRVIECLQNST--------NGVLESPTGTGK 48 Tribolium castaneum 1 MTTITIRGVPVKFP--FAPYDIQTKYMEKVIDCLENRQ--------NGILESPTGTGK 48 Nematostella vectensis 1 MVVVRKVDVDFP--FKPYDCQVAYMEKVIECLQTRK--------NAVLESPTGTGK 46

  2. Fig. S1B, C B Q I HsFANCJ 1 MSSMWSEYTIGGVKIYFPY-KAYPSQLAMMNSILRGLNSKQH—-CLLESPTGSGKSLALLCSALA 62 HsRTEL1 1 MPKIVLNGVTVDFP-FQPYKCQQEYMTKVLECLQQKVN—-GILESPTGTGKTLCLLCTTLA 58 MmRTEL 1 MPRVVLNGVTVDFP-FQPYPCQQEYMTKVLECLQKKVN—-GILESPTGTGKTLCLLCSTLA 58 CeRTEL-1 5 ASNSTEVWINPKLSVKFP-FEPYECQRIFMKNVVDVLDRKLD—-AALESPTGTGKTLSLLCSTLA 66 HsChIR1 1 MANETQKVGAIHFPFP-FTPYSIQEDFMAELYRVLEAGK—-IGIFESPTGTGKSLSLICGALS 60 MmChIR1 1 MADENQEIGGIHFPFP-FPPYPIQKDFMAELYKVLEGGK—-IGIFESPTGTGKSLSLICGALS 60 CeCHL-1 1 MDEFSFP-FQPYDIQLNLMREIRQCIEQRK—-IGIFESPTGTGKSLSVLCSTMT 51 ScChI1 1 MDKKEYSETFYHP-YKPYDIQVQLMETVYRVLSEGKK-IAILESPTGTGKTLSLICATMT 58 HsXPD 1 MKLNVDGLLVYFPYDYIYPEQFSYMRELKRTLDAKGH—-GVLEMPSGTGKTVSLLALIMA 58 MmXPD 1 MKLNVDGLLVYFPYDYIYPEQFSYMLELKRTLDAKGH—-GVLEMPSGTGKTVSLLALIVA 58 CeXPD-1 1 MQLDIDGLKVLFPYDYVYPEQVLYMKEVKKALDARGH—-GLLEMPSGTGKTVSLLSLVLA 58 ScXPD 1 MKFYIDDLPVLFPYPKIYPEQYNYMCDIKKTLDVGGN—-SILEMPSGTGKTVSLLSLTIA 58 ScRAD3 1 MKFYIDDLPVLFPYPKIYPEQYNYMCDIKKTLDVGGN—-SILEMPSGTGKTVSLLSLTIA 58 EcoliDinG 7 LKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAGEEGRHLAIEAPTGVGKTLSYLIPGIA 70 C 0 or Q I E.Coli RECQ 1 MNVAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSG-RDCLVVMPTGGGKSLCYQI 61 RECQ1 63 SSPAAWNKEDFPWSGKVKDILQNVFKLEKFRPLQLETINVTMAG-KEVFLVMPTGGGKSLCYQL 125 WRN 524 EEDDDKDFLWPAPNEEQVTCLKMYFGHSSFKPVQWKVIHSVLEERRDNVAVMATGYGKSLCFQY 587 RECQ4 95 PLYSLGPSGQLAETPAEVFQALEQLGHQAFRPGQERAVMRILSG-ISTLLVLPTGAGKSLCYQL 157 RECQ5 1 MSSHHTTFPFDPERRVRSTLKKVFGFDSFKTPLQESATMAVVKGNKDVFVCMPTGAGKSLCYQL 64 BLM 628 LKHERFQSLSFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLG-EDCFILMPTGGGKSLCYQL 690 Q672R (BLM)

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