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Differentiate the extraction method used to extract metabolic compounds, protein & nucleic acid. Include the principle of extraction, common method &equipment used in each extraction process.
Two kinds of polymer or a kind of polymer and a kind of salt dissolved in the same solvent, because of the Non-Intersoluble between polymers or polymer and salt, when the concentration of polymer or inorganic salt reached a certain value, will divided into two phase which are Non-Intersoluble.
Principle of Extraction
Aqueous Two Phase Extraction is a method of extraction that utilize the materials which has got a different partition coefficient in two insoluble, aqueous two-phase.
The system that can form aqueous two-phase are:
Ion-type polymer - non-ion-type polymer(intermolecular repulsion)
polymer-low molecular weight compound(salting-out effect)
－the effect of interfacial tension to mass transfer is much greater than those other physical properties, for instance: density, viscosity, diffusion coefficient.
PRINCIPLE OF CONVENTIONAL EXTRACTION SYSTEM
Aqueous solvent system
Non- aqueous solvent system
Process Flow Diagram