A Comparative Study of Methicillin Resistant Staphylococcus aureus Nasal Carriage Rates Between Veterinarians and Veterinary Technicians Diane Hartman, DVM Tamarah Adair, PhD Amanda Hartman, BS Purpose 1. Determine the prevalence of MRSA carriage among veterinarians,
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A Comparative Study of Methicillin Resistant Staphylococcus aureusNasal Carriage Rates Between Veterinarians and Veterinary Technicians
Diane Hartman, DVM
Tamarah Adair, PhD
Amanda Hartman, BS
1. Determine the prevalence of MRSA carriage among veterinarians,
veterinary technicians and others
at a Fall 2008 veterinary conference.
4. Distinguish between HA-MRSA and CA-MRSA based on PCR and gel electrophoresis.
25-30% of the population harbors Staphylococcus aureus in their nasal passages
1-3% carry MRSA.
skin and soft tissue infections
source of exposure
PCR/Pulse field gel electrophoresis
Prolonged antibiotic use
Cases arise sporadically
At risk groups
contact sports participants
usually susceptible to many different antibiotics
determine possible sources of exposure
recent surgery or hospitalization
volunteer in hospital, day care, nursing home
analyze common risk factors
travel, antibiotic use, recent skin infection, public gym, contact sports
JMP computer program was used to evaluate potential risk factors
Consent forms - required for each participant
Nasal swabs to
mannitol salt agar
Identify Mannitol fermenters that are Gram + cocci
Oxacillin MIC/E Strips
CCR-all ccr type 2
16 were type 2 ccr.
3 were Class A mec
13 were Class B mec
Class A mec with type 2 ccr are Type II - HA strains
Class B mec with type 2ccr are Type IV -CA strains
Potential Risk Factors for Technicians?
1. Spend more time in the exam room with
clients and pets
2. Spend more time with their face close to the
3. Spend more time face to face with clients in
the exam room and waiting area
4. More likely to interact with client’s children
5. More likely to perform dental prophys on pets
6. More likely to clean cages/kennels
7. More likely to clean ears and/or bathepets
WASH YOUR HANDS!
Don’t share towels or other personal items.
Cleanse fresh wounds and keep wounds covered.
Wear appropriate PPE for the task,
e.g. dentals - lab coats, gloves, mask/face shield.
Supported by the Baylor Undergraduate Research and Scholarly Activities Small Grant Program
and the Vice Provost for Research
Department of Biology Baylor University
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