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WT …TGTAGGGCTG… mSA …TGTctGGCTG… - PowerPoint PPT Presentation


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LUC. pA. K30-3'-asLUC. K30-3'-asLUC mSA. A. 900. B. e1. intron. exon 2. M. 750. 3’ LTR. 600. Luciferase activtiy (RLU). WT …TGTAGGGCTG… mSA …TGTctGGCTG…. 450. *. 300. 4. 5. 6. 1. 2. 3. 150. -. -. RT enzyme. +. +. +. +. 0. RT primer. -. +. -. +. -. -.

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Presentation Transcript

LUC

pA

K30-3'-asLUC

K30-3'-asLUC mSA

A

900

B

e1

intron

exon 2

M

750

3’ LTR

600

Luciferase activtiy (RLU)

WT …TGTAGGGCTG…

mSA …TGTctGGCTG…

450

*

300

4

5

6

1

2

3

150

-

-

RT enzyme

+

+

+

+

0

RT primer

-

+

-

+

-

-

K30-3'-asLUC

K30-3'-asLUC mSA

C

WT …TGGATGGCGG…

mATG/e1 …TGGAacGCGG…

WT …AGCATGGTTA…

mATG/int …AGCcTaGTTA…

intron

exon 2

e1

WT …TAGGTAGGCT…

mSD2 …TAGcaAGGCT…

WT …CAGGTAGGGC…

mSD1 …CAGcaAGGGC…

WT …TGTAGGGCTG…

mSA …TGTctGGCTG…

D

SP1

mSA

WT

HBZ

mSD2

mSD1

mATG/e1

mATG/int


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