Metabolism ( all the chemical reactions occur in a cell )

1. Catabolism ( breaking down of molecules ) - usually releases energy Metabolism ( all the chemical reactions occur in a cell ) Aanabolism ( synthesis of molecules ) - usually requires energy

2. Amino acids may not be linked together but are close distance to each other Other amino acids for maintaining the correct globular structure

4. E + S ES complex EP complex E + P

7. Differences LKH IFH may not be exactly right in shape to fit the substrate 1. Initial shape of active site exactly fit with substrate 2. Flexibility of enzyme molecule more less 3. Change in shape of active site yes, induced by the binding of substrate with active site no Compare and contrast the “lock and key hypothesis” and “induce fit hypothesis” Similarities : 1. Similar reaction sequence : E+S ->ES complex ->EP complex ->E+P 2. Catalytic reaction occurs in active site of enzyme

8. Activator (inorganic )

10. B C A

11. Factor : pH Temperature enzyme Conc Substrate Conc Coenzyme Conc . . Rate of enzymatic reaction Which is the limiting factor which controls the rate of reaction ?

12. For fixed amount of substrate

14. Competitive inhibitor Non competitiveinhibitor