Nitric Oxide Synthase Joey Klen. Introduction. Nitric oxide (NO) is an important signaling biomolecule and a cytotoxin. NO is produced by nitric oxide synthase (NOS) Neuronal ( nNOS ) Neuronal tissues; NO as a neurotransmitter Inducible ( iNOS ) Macrophages; NO as a cytotoxin
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L-citrulline and NO
Heme Binding Residues
L-Arg/NHA Binding Residues
H4B Binding Residues
Zn Tetrathiolate Cys Residues
A. Rate of NO production of WT rat nNOS monitored by Hb assay at room temperature.
B. Lineweaver-Burk plot for dtermination of Km and Vmax.