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Case Study on Human Papillomavirus. Teresa Dominguez LaTasha Hardy Francisca Mata. Case Study Overview. 25-five-year-old carpenter. Several hyperkeratotic papules (warts) on Palm inside of index finger. Do not change size. Cause only minimal discomfort.

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Case Study on Human Papillomavirus

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Case study on human papillomavirus

Case Study on

Human

Papillomavirus

Teresa Dominguez

LaTasha Hardy

Francisca Mata


Case study on human papillomavirus

Case Study Overview

  • 25-five-year-old carpenter

  • Several hyperkeratotic papules (warts) on

  • Palm inside of index finger

  • Do not change size

  • Cause only minimal discomfort

  • Spontaneously disappear after a year


Case study on human papillomavirus

What are Warts?

Non-cancerous skin growths in the epidermis

Caused mostly by HPV-1, 2 and 4

Usually skin-colored and feel rough to the

touch

Several types:

Common warts

Foot (planar) warts

Flat warts


Case study on human papillomavirus

Fig. 1 HPV infecting the

epidermis

Fig. 2 More extreme case of

common warts.


Case study on human papillomavirus

Question I

Will this virus infection spread to

other body parts?

Transmission via contact

Infection of basal cells of epidermis

Breach in the skin predisposing factor

There is a possibility of spreading warts to other parts of your body through breaks in the skin.


Case study on human papillomavirus

Question II

After its disappearance, is the infection

likely to be completely resolved

or persist in the host?

Immunocompetent persons:

Once infected with a specific HPV type,

it is unlikely

Immunosuppressed persons:

Can present with numerous treatment

resistant warts

Recurrent infections with same HPV type


Case study on human papillomavirus

Question IIIWhat viral, cellular, and host conditions regulate the replication of this virus and other HPV’s?

  • The Virus

    • icosahedral particle, 72 capsomers

    • Closed, circular dsDNA

computer colorized EM image of Papillomavirus capsid

Genomic organization of HPV-16


Dna replication

DNA Replication

  • Replicates and assembles in the nucleus

  • Dependent on:

    • transcription is tightly regulated by the differentiation state of the infected epithelial cell


Dna replication1

DNA Replication


Qiv how would the papilloma type causing this infection be identified

QIV:How would the papilloma type causing this infection be identified?

What is known


Detection methods

Detection Methods

  • Clinical Recognition

  • PCR and RT-PCR

  • PCR Cloning and Sequencing

  • Standard enzyme linked immunosorbent assay (ELISA)

  • Histological Analysis


Qv is it likely that this type of hpv is associated with human cancer

QV: Is it likely that this type of HPV is associated with human cancer?

This type of HPV is not associated with human cancer

HPV-2 (hand warts)

Common skin wart

Does not cause genital warts

Not associated with development of cancer


Which types of hpv are associated with cancer

Which types of HPV are associated with cancer?


Case study on human papillomavirus

15 classified as “high-risk”

HPV Strains:

  • 16,18,31,33,35,39,45,51,52,56,58,59,68,73, &82

  • 16 & 18 cause 70% of cervical cancers

  • 2 types of cervical cancer (squamous cell cancer and adenocarcinoma)


Case study on human papillomavirus

HPV and Cancer

  • Anal cancer, vulvar cancer, penile cancer, and throat cancer

  • 3 classified as probable high-risk (HPV-26,53, &66)

  • 12 classified as “low-risk” (HPV-6,11,40,42,43,44,54,61,70,72,81, &CP6108)


Hpv induced cancers

HPV- Induced Cancers

http://HPV_tree_1.png


Case study on human papillomavirus

References

Antonsson, A, et.al. The Ubiquity and Impressive Genomic Diversity of Human Skin Papillomaviruses Suggest a Commensalic Nature of These Viruses. J. Virol. 2000. Vol. (74)

Centers for Disease Control and Prevention. 2007 Human Papillomavirus: HPV Information for Clinicians (Brochure). Washington, DC. April 2007

Desante, C., and Demeret, C. Control of papillomavirus DNA replication and transcription. Seminars in Cancer Biology, 1996: (51): 339–347

Kari, I. et. al. Antisense RNA directed to the human papillomavirus type 16 E7 mRNA from herpes simplex virus type 1 derived vectors is expressed in CaSki cells and downregulates E7 mRNA. J. Virol. 2007, 4:47

Kingsley K, Johnson D, O’Malley, S. Transfection of oral squamous cell carcinoma with human papillomavirus-16 induces proliferative and morphological changes in vitro. Cancer Cell Int. 2006 May 22;6:14

Lambert, P. Papillomavirus Replication. J. Virol. 1991, (65):3417-3420.

Lehtinen, M. Serologically diagnosed infection with human papillomavirus type 16 and risk for subsequent development of cervical carcinoma: nested case-control study. BMJ 1996;(312):537-539

McBride AA, Romanczuk H, Howley PM. The Papillomavirus E2 Regulatory Proteins. J Biol Chem 1991 Oct. 266(28); 18411-18414

Mino T, Mori T, Aoyama Y, Sera T. Development of protein-based antiviral drugs for human papillomaviruses. Nucleic Acids Symp Ser (Oxf).2007; (51):427-8

Reddout, N. et. al. High Risk HPV types 18 and 16 are potent modulators of oral squamous cell carcinoma phenotypes in vitro. Infect Agent Cancer. 2007 Nov. 14;2(1):21

Stanley, MA. et. al. HPV: From infection to cancer. Biochem Soc Trans. 2007 Dec;35(Pt 6): 1456-60.

Walboomers, JM, Jacobs MV, Manos MM, et. al. Human papillomavirus is a necessary cause of invasive cervical cancer worldwide. J Pathology. 1999 Oct. 189(1):12-9

Werness BA, Levine AJ, Howley PM. Association of human papillomavirus types 16

and 18 E6 proteins with p53. Science 1990 Apr 6; 248(4951):76-9.

http://en.wikipedia.org/wiki/Human_papillomavirus

http://www.gsbs.utmb.edu/microbook/ch066.htm

http://www.healthscout.com/ency/68/384/main.html

http://www.medicinenet.com/genital_warts_in_women/article.htm

http://www.oncolink.upenn.edu/types/article.cfm?c=6&s=17&ss=131&id=9531

http://www.oralcancerfoundation.org/facts/humanpapillomavirus.htm


Case study on human papillomavirus

THANK YOU!


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