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ÁCIDOS NUCLÉICOS

ÁCIDOS NUCLÉICOS. Nucleotídeo Unidade Formadora de Ac. Nucléico. Bases Nitrogenadas : Purínicas: Adenina Guanina Pirimidínicas: Citosina Timina Uracila. Tipos: DNA (Ac. Desoxirribonucléico) RNA (Ac. Ribonucléico ). C T G T C A C T C G G G T. G A C A G T G A G C C C A.

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ÁCIDOS NUCLÉICOS

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  1. ÁCIDOS NUCLÉICOS

  2. Nucleotídeo • Unidade Formadora de Ac. Nucléico

  3. Bases Nitrogenadas: • Purínicas: • Adenina • Guanina • Pirimidínicas: • Citosina • Timina • Uracila

  4. Tipos: DNA (Ac. Desoxirribonucléico) RNA (Ac. Ribonucléico)

  5. CTGT C A CTCG G GT G A C AG TG A G C C C A DNA: ESTRUTURA GERAL

  6. DNA: ESTRUTURA DETALHADA

  7. AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT Se a informação genética contida em cada célula nucleada fosse escrita em um livro,haveriam aproximadamente 500.000 páginas escritas de ambos os lados

  8. CTGT C A CTCG G GT G A C AG TG A G C C C A DNA: ESTRUTURA GERAL

  9. FLUXO DA EXPRESSÃO GÊNICA DNA RNA PROTEÍNA DNA

  10. REPLICAÇÃO: • Biossíntese de DNA à partir • de um molde de DNA • Ocorre na fase S • Ocorre no sentido 5’ – 3´ • Semi-conservativo

  11. Começa em sítios específicos (Ricos em A e T • Eucarioto: ~30.000sítios por cromossoma • Procarioto: Único sítio (OriC)

  12. Replicação: • Contínua 5’ –3’ • Descontínua (Fragmentos de OKASAKI)

  13. COMPONENTES: • Helicase • SSB • Primer • Primase • DNA Polimerase SIGMA • Topoisomerase • DNA Ligase

  14. PCR (Polymerize Chain reaction) • Replicação in vitro • Aplicação: Diagnóstico molecular de Doenças Genéticas e Infecciosas

  15. CONDIÇÔES DA PCR • Temperatura: • Desnaturação : 95o C • Anelamento: 60oC • Corrida: 72oC • Vários Ciclos: 30 • Enzima: Taq Polimerase

  16. ...T T C G A T G G A T A A A C C G A... ...A A G C T A C C T A T T T G G C T... Reação em Cadeia da Polimerase

  17. ...T T C G A T G G A T A A A C C G A... A A G C ...A A G C T A C C T A T T T G G C T... C C G A Reação em Cadeia da Polimerase

  18. ...T T C G A T G G A T A A A C C G A... ...A A G C T A C C T A T T T G G C T... ...T T C G A T G G A T A A A C C G A... ...A A G C T A C C T A T T T G G C T... Reação em Cadeia da Polimerase

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