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酶工程. 生物技术 的 四大支柱. 酶的基础知识. 一 什么是酶?. Enzyme proteins 酶是由生物体产生的具有催化剂活性的蛋白质 。 Ribozyme RNAs 本身就是一段 RNA ,不需要额外的蛋白酶就可以对自身进行剪切。. 二 酶催化特性. 高效率 比非催化高 10 8 - 10 20 倍 比非酶催化高 10 7 - 10 13 倍 高度专一性 反应条件温和 酶催化是可调控的. 三 酶的化学本质. Enzyme Proteins Ribozyme RNAs. 四 酶的组成. 简单酶 酶 结合酶

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酶工程

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  • Enzyme proteins

  • Ribozyme RNAs

    • RNA


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    • 1081020

      1071013


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    • Enzyme Proteins

    • Ribozyme RNAs


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    Coenzyme

    Prosthetic group (Table 2)

    Holoenzyme

    Apoenzyme

    Cofactor


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    • Ribonuclease

      Protease

    • Dehydrogenase

      Phosphotransferase

      Aminotransferase


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    • Oxidoreductases

    • Transgerases

    • Hydrolases

    • Lyases

    • Isomerases

    • Synthetases

      Synthases


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    • ATP: Creatine Phosphotransferase

      ATP:

    • Glutamate: Pyruvate aminotransferase

      (GPT)

    • Monomeric enzymes

    • Oligomericenzymes

    • Multienzyme systems


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    1

    2

    3

    4

    5


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    • 19

      (1)

      (2)4000


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    • 1987Bucher


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    • Takamine


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    Pilot Biostat UD50


    20 90

    2090


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    (1)

    (2)

    (3)


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    (4)


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    ()


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    ()


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    ()

    (1)

    (2)


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    ()


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    ()

    (1)

    (2)

    (3)

    (4)

    (5)

    (6)

    (7)


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    ()

    (1)

    (2)

    (3)()

    (4)


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    ()

    (1)


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    ()

    (2)


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    ()

    (3)


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    ()

    (1)

    (2)


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    ()

    (1)

    (2)

    (3)


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    • One order self-catalytic model


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    ,,,,(PCL),,,AS1398 ,,601 ,,


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    • SARSSARSAG7088SARSrhinovirusSARSAG7088SARSSARSSARS


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    , , ,


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    ..,6-. , , ., .


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    1. ()2. ()3. L-()


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    D


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    • DEAE Cellulose

    • DEAE-Sephadex A-50


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    • Enzyme+N, N-,

    • inactiation


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    O O

    H C CH2 CH2 CH2 C H


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    • 711

    • ab


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    72

    1316

    221718

    4433 19

    4422 20

    1524 21

    24 22

    344 23


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    -DL Ala L Ala +

    -D Ala

    Aminoacylase


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    A-L-Ala

    A-D-Ala

    L-Ala A-D-Ala

    L-Ala


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    1

    DO2 D15H2O


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    H2O

    Pt

    2H2e


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    2

    Urea + 2H2O 2NH4++2HCO3-

    2NH4+

    NH3


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    GdnHCl

    GdnHCl

    C

    B

    A

    Creatine Kinase

    MW 82000 Dimer


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    73

    g/ml /mg)

    A400 10028.0

    B210 52.525.7

    C380 95.027.4


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