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酶工程. 生物技术 的 四大支柱. 酶的基础知识. 一 什么是酶?. Enzyme proteins 酶是由生物体产生的具有催化剂活性的蛋白质 。 Ribozyme RNAs 本身就是一段 RNA ,不需要额外的蛋白酶就可以对自身进行剪切。. 二 酶催化特性. 高效率 比非催化高 10 8 - 10 20 倍 比非酶催化高 10 7 - 10 13 倍 高度专一性 反应条件温和 酶催化是可调控的. 三 酶的化学本质. Enzyme Proteins Ribozyme RNAs. 四 酶的组成. 简单酶 酶 结合酶

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酶工程

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  • Enzyme proteins

  • Ribozyme RNAs

    • RNA


    • 1081020

      1071013


    • Enzyme Proteins

    • Ribozyme RNAs


    Coenzyme

    Prosthetic group (Table 2)

    Holoenzyme

    Apoenzyme

    Cofactor


    • Ribonuclease

      Protease

    • Dehydrogenase

      Phosphotransferase

      Aminotransferase


    • Oxidoreductases

    • Transgerases

    • Hydrolases

    • Lyases

    • Isomerases

    • Synthetases

      Synthases


    • ATP: Creatine Phosphotransferase

      ATP:

    • Glutamate: Pyruvate aminotransferase

      (GPT)

    • Monomeric enzymes

    • Oligomericenzymes

    • Multienzyme systems




    1

    2

    3

    4

    5


    • 19

      (1)

      (2)4000


    • 1987Bucher


    • Takamine



    Pilot Biostat UD50


    2090


    (1)

    (2)

    (3)


    (4)


    ()


    ()


    ()

    (1)

    (2)


    ()


    ()

    (1)

    (2)

    (3)

    (4)

    (5)

    (6)

    (7)


    ()

    (1)

    (2)

    (3)()

    (4)


    ()

    (1)


    ()

    (2)


    ()

    (3)


    ()

    (1)

    (2)


    ()

    (1)

    (2)

    (3)




    • One order self-catalytic model





    ,,,,(PCL),,,AS1398 ,,601 ,,


    • SARSSARSAG7088SARSrhinovirusSARSAG7088SARSSARSSARS




    , , ,


    ..,6-. , , ., .


    1. ()2. ()3. L-()








    D




    • DEAE Cellulose

    • DEAE-Sephadex A-50


    • Enzyme+N, N-,

    • inactiation


    O O

    H C CH2 CH2 CH2 C H


    • 711

    • ab


    72

    1316

    221718

    4433 19

    4422 20

    1524 21

    24 22

    344 23



    -DL Ala L Ala +

    -D Ala

    Aminoacylase


    A-L-Ala

    A-D-Ala

    L-Ala A-D-Ala

    L-Ala


    1

    DO2 D15H2O


    H2O

    Pt

    2H2e


    2

    Urea + 2H2O 2NH4++2HCO3-

    2NH4+

    NH3


    GdnHCl

    GdnHCl

    C

    B

    A

    Creatine Kinase

    MW 82000 Dimer


    73

    g/ml /mg)

    A400 10028.0

    B210 52.525.7

    C380 95.027.4



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