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The Role of the Histology Professional

The Role of the Histology Professional. The National Society for Histotechnology. MAKING A DIFFERENCE. HISTOTECHNOLOGY BENEFITS MANKIND. Histotechnology Professionals in the marine and veterinary world reveal the microscopic secrets that enable scientists to keep our food safe.

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The Role of the Histology Professional

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  1. The Role of the Histology Professional The National Society for Histotechnology

  2. MAKING A DIFFERENCE The National Society for Histotechnology

  3. HISTOTECHNOLOGY BENEFITS MANKIND • Histotechnology Professionals in the marine and veterinary world reveal the microscopic secrets that enable scientists to keep our food safe. • Histotechnology Professionals who work in the toxicology lab enable scientists to understand how toxins pollute our environment. The National Society for Histotechnology

  4. HISTOTECHNOLOGY BENEFITS MANKIND • Histotechnology Professionals in the pharmaceutical industry enhance our lives and increase longevity. Their work reveals the effectiveness of medication for our health. • Histotechnology Professionals who work in the clinical environment work closely with Pathologists to investigate and determine disease in Humans. The National Society for Histotechnology

  5. SO WHAT EXACTLY DOES A HISTOLOGY PROFESSIONAL DO? • Process tissue • Embed tissue • Cut tissue • Stain tissue The National Society for Histotechnology

  6. TISSUE PROCESSORINFILTRATES THE TISSUE WITH WAX Need a picture of a tissue processor The National Society for Histotechnology

  7. Histology Professional orienting the tissue in the embedding mold The National Society for Histotechnology

  8. Histology Professional embedding and chilling tissue into a wax block The National Society for Histotechnology

  9. The Histology Professional cutting the paraffin wax block with the tissue inside it. This is cut in very thin slices and placed on a glass microscope slide. The National Society for Histotechnology

  10. The Histology Professional cutting the paraffin wax block with the tissue inside. This is cut in very thin slices and placed on a glass microscope slide. The National Society for Histotechnology

  11. The Histology Professional then floats the thin wax sections of tissue onto a waterbath, and picks them up from there on the glass slide The National Society for Histotechnology

  12. Now the tissue is on the slide. But… it is transparent and must be stained in color to be seen by the Pathologist. The National Society for Histotechnology

  13. Once slides are stained, the tissue reveals itself through cellular structure. Pathologists can determine abnormalities and diagnose disease. The National Society for Histotechnology

  14. Slides are warmed in an oven to melt the paraffin and make sure the tissue adheres to the slide during staining The National Society for Histotechnology

  15. Slides are stained most often by an automatic staining machine. The National Society for Histotechnology

  16. Following staining, the slide is covered with a thin piece of glass called a ‘coverslip’. The National Society for Histotechnology

  17. The coverslip is mounted with a permanent resin. This makes the slide stronger and permanent- protecting the stained tissue for years. The National Society for Histotechnology

  18. The Pathologist then examines the slides to determine the disease and diagnosis. The National Society for Histotechnology

  19. PLANT PATHOLOGISTS STUDY DISEASED PLANT TISSUE The National Society for Histotechnology

  20. PLANT PATHOLOGISTS STUDY DISEASED PLANT TISSUE Interested in a Career in Histology? Visit www.NSH.org for more information on educational requirements The National Society for Histotechnology

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