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Organogenesis in Peanut
Research Experience for Undergraduates
Food Biotechnology Laboratory
Alabama A&M University
What is tissue culture?
Why is it important?
How can tissue culture be applied to peanut improvement?
Tissue culture - is the culture and maintenance of plant cells and organs.
Important parameters in tissue culture
- Type of explants: leaf, stem, hypocotyl, root, petiole, etc.
Macro nutrient and Micronutrients
Cytokinins - TDZ, BAP
Auxins - NAA
- Aseptic technique
Plant tissue culture has value in studies such as: cell biology, genetics, biochemistry, and many other research areas.
Plants can be produced quickly
Plantlets can be used for germplasm conservation
Relies on the production of organs either directly from an explant or callus structure
Embryo-like structures which can develop into whole plants in a way that is similar to zygotic embryos are formed from somatic cells
Uses meristematic cells to regenerate whole plant.
(Source:Victor. et al., 2004)
(Source:Victor. et al, 2004)
Importance of Peanut
Current status of peanut organogenesis
To compare peanut regeneration through organogenesis using different hormones and hormones at different concentrations.
(. Source: Li. et al, 2003)
Fig 1. Flow Diagram for peanut regeneration
TDZ (Thidiazuron) : 10uM, 15uM, 20uM
BAP (Benzylamineopunine): 10uM, 15uM, 20uM
HA (Humic Acid) :: 12.5 mg/L, 25 mg/L, 50mg/L
*make sure seeds contain no fungi or bacteria*
(Source:Victor.et al, 2004)
Fig. 5 Steps in peanut regeneration
Germination using 3 different hormones at 3 different concentrations
Cut hypocotyl and reculture in same hormone concentration.
- vitamins and
- enzymatic poisons
on peanut germination and regeneration.