Jugular blood collection in a conscious rat
Download
1 / 17

Jugular Blood Collection in a Conscious Rat - PowerPoint PPT Presentation


  • 733 Views
  • Uploaded on

Jugular Blood Collection in a Conscious Rat. Calm the rat down before restraining by gently stroking the animal. Restrain the rat by grasping the skin over the nape of the neck and shoulder region with your thumb and index finger (scruffing).

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about 'Jugular Blood Collection in a Conscious Rat' - Sophia


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript


Restrain the rat by grasping the skin over the nape of the neck and shoulder region with your thumb and index finger (scruffing).


Once you have scruffed the neck, gently turn your hand over so the rat is lying comfortly in the palm of your hand. Do not squeeze the rat around the thorax region because that could cause difficulty breathing. Make sure the rat’s body is straight and not bent or curled in your palm.


With your other hand, use index finger to lightly hold head out of way. Do not put to much pressure on rat’s head or neck. With thumb, hold forelimbs back, while another technician shaves the area.



With your other hand, use index finger to lightly hold head. Do not put to much pressure on rats head or neck. With thumb, apply slight pressure on vein proximal to the collection site so it will distend. This will make the jugular vein more prominent for easier visualization. The bottom half of rat is still just resting in palm of hand.


Have the technician drawing blood get the syringe and needle ready. We use a 1 ml syringe with a 23 gauge needle.


Insert needle through skin right around the middle point between the sternum and shoulder area, where you should see the vein. Advance slowly. Create a slight vacuum within the syringe, while advancing your needle.


Once you hit the vein, a small drop of blood will leak into the hub of the needle. Try not to let your syringe move around and keep drawing back on the plunger. Do not pull back too fast because it might cause the vein to collapse.


Once you have obtained desired amount of blood, apply a slight amount of pressure to the puncture site.


ad