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Genetic Diversity of Stream Fish Populations in the Mid-Atlantic Region. Suzanne Christ, Mark Bagley, Frank McCormick US Environmental Protection Agency, National Exposure Research Laboratory Cincinnati OH. What does genetic diversity have to do with vulnerability assessments?.

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Genetic diversity of stream fish populations in the mid atlantic region l.jpg

Genetic Diversity of Stream Fish Populations in the Mid-Atlantic Region

Suzanne Christ, Mark Bagley, Frank McCormick

US Environmental Protection Agency,

National Exposure Research Laboratory

Cincinnati OH


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What does genetic diversity have to do with vulnerability assessments?

ReVA Endpoints:

  • Resource Productivity

  • Clean Drinking Water

  • Biodiversity


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What does genetic diversity have to do with vulnerability assessments?

ReVA Endpoints:

  • Resource Productivity

  • Clean Drinking Water

  • Biodiversity


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Ecosystem assessments?

Species

Genes

Biodiversity


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Genetic Diversity assessments?

  • Variation in the heritable portion of measurable traits that exists among individuals within a species.

    • Genetic diversity within populations

    • Genetic diversity among populations

  • Examples: eye color, height, blood type, HIV resistance


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Δ assessments? Environment Δ Genetic Diversity Δ Vulnerability

 Inbreeding depression

 Heterozygosity

 Adaptation

( Environment tracking)

 Genetic Drift

 Inbreeding

 Population size

 Density

 Replacement rate variability

 Ne

 Demographic randomness

Gilpin and Soule’s (1987) Extinction Vortex Model (Adaptation and inbreeding vortices)

EXTINCTION



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Spatial distribution of genetically distinct resource populations (ESUs)

  • Unique resources

  • Biodiversity “Hotspots”



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Relative vulnerabilities of populations (ESUs)populations


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What does genetic diversity have to do with vulnerability assessments?

  • Indicator of population’s future “environmental buffering” ability

  • Reflective of past population stressor history

  • Defines the fundamental biological unit that we wish to assess (the population)

    • Prioritization based on uniqueness and vulnerability


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Genetics of Creek Chubs in a assessments?Coal Mining Region of the Mid-Atlantic

Semotilus atromaculatus

Photo courtesy of Ohio Dept. Natural Resources


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Study Questions assessments?

  • Are there distinct population resources in the MAIA region?

  • Do watershed boundaries (HUCs) predict stream population boundaries?

  • Do levels of genetic diversity differ among sites?

  • What is the relationship between genetic diversity and environmental condition?


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Study Area assessments?

  • Five watersheds assessed in MAIA pilot

  • 10 sample sites

  • Part of EMAP-MAIA 97-98 sampling

  • Wadeable streams (second and third order)



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Genetic Analyses assessments?

  • 10-28 fish sampled per site

  • 590 bp of mitochondrial Cyt-B gene sequenced

  • AFLP fingerprints based on 109 polymorphic markers

  • Genetic differences within and among sites assessed by Analysis of Molecular Variance


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Population assessments?

genetic

structure

Mitochondrial DNA


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Population assessments?

genetic

structure

Nuclear DNA


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Partitioning of Genetic Variance: assessments?

Mitochondrial DNA

Among

Regions

64.8%

Among Sites

Within Sites

Within Regions

27.8%

7.4%


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Partitioning of Genetic Variance: assessments?

Nuclear DNA

Within Sites

86.6%

Among

Among Sites

Regions

Within Regions

5.4%

8.0%


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Study Questions assessments?

  • Are there distinct population resources in the MAIA region? YES

  • Do watershed boundaries (HUCs) predict stream population boundaries? NOT 1:1

  • Do levels of genetic diversity differ among sites?

  • What is the relationship between genetic diversity and environmental condition?


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Average Genetic assessments?Similarity




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Stepwise multiple regression assessments?nuclear DNA diversity


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Study Questions assessments?

  • Are there distinct population resources in the MAIA region? YES

  • Do watershed boundaries (HUCs) predict stream population boundaries? NOT 1:1

  • Do levels of genetic diversity differ among sites? YES

  • What is the relationship between genetic diversity and environmental condition? APPEARS TO BE STRONG


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Complete Study assessments?

  • 3 stream minnows

  • MAIA and WAP

  • To be completed

  • later this year


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More info: [email protected]

513 569 7455

Acknowledgements

Betsy Smith (ReVA)

Tony Leonard (Sobran, Univ. of Cincinnati)

EMAP program


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