Biotechnology: Status and Uses. Animal Science 434 John J. Parrish. Reproductive Biotechnology's. Artificial insemination In vitro embryo production In vivo embryo production Embryo transfer Gender selection Stem Cells Genetic engineering Cloning. In Vitro Production of Embryos.
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Biotechnology: Status and Uses
Animal Science 434
John J. Parrish
Day 6 or 7
Tight Morula (day 5 - 7)
Early Blastocyst (day 7 - 8)
Blastocyst (day 7 - 9)
Octopus Springs - Yellowstone National Park
Home of Thermus aquaticus - Taq Polymerase
F M F F F F F F
Selection of X or Y sperm
X Chromosome has more DNA!!!
Flow Cytometer Separation of X and Y Sperm
Cycles are limited
Only 3 - 4 cycles
Cells of InterestGrown in Culture
Mature Oocyte enucleated and cell of interest fused
Inner Cell Mass Cells isolated and grown in culture
Less than 1% efficiency
Better success but left with potential for viral replication