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Biological Safety Training
modified slightly from REM 2008
…………. to biohazards can occur by
Intact skin is regarded as an effective barrier.
Biosafety levels corresponds to the rating of the biohazard, i.e.,
BSL2 required for RG 2
(BSL2 is the most common type of biohazard project that occurs on campus.)
(if aerosol production is anticipated).
Respiratory Protection comes in many varieties. Choose the appropriate mask for the task.
BIO-RELATED WASTE MATERIALS:
Protect yourself from the contents.
Hold Bio bags away from your body.
Don’t squeeze the bag so aerosols are released into your breathing space.
Take biowaste to the autoclave room as soon as possible.
- Sealed containers - Aerosol cans
- Volatiles - Uncontained sharps
DO NOT PLACE SHARPS CONTAINERS IN THE REGULAR TRASH!
CALL REM FOR PICKUP
ALWAYS place sharps in puncture resistant containers for disposal.
In the event of a spill:
Use the proper PPE
Use the proper disinfectant solution.
Dispose of clean-up materials in biohazard bags.
Special security measures apply to users of “Select Agents.” Contact the University Biosafety Officer for more specific details.
Biological Safety Cabinet Laminar Flow Clean Bench
HEPA stands for:
Biological Safety Cabinets
Laminar Flow Clean Benches
HEPA filters on both supply and exhaust
HEPA filtered supply air only
BSC – Safe for Biohazard Use
BIOSAFETY CABINET AIRFLOW
(Courtesy of Eagleson Institute)
POTENTIALLY CONTAMINATED AIR
HEPA FILTERED AIR
Courtesy of Eagleson Institute
No one at Purdue has been approved for Select Agents at this time.
Center For Disease Control and Prevention
Select Agent Program
Biosafety in Microbiological and Biomedical Laboratories 5th Edition, January 20007
Material Safety Data Sheets For Research Pathogens
Purdue University Biosafety Manual
Guidelines for Research Involving Recombinant DNA Molecules (NIH Guidelines)
Know the Risks!
If you can anticipate facing contact with blood and/or other potentially infectious materials as part of your job duties you should receive Bloodborne Pathogen awareness training.http://www.purdue.edu/REM
Wash hands for 20 seconds after removing gloves or immediately after an exposure.
Use non-abrasive soap.
Must be placed in closeable, leak-proof containers built to contain all contents during handling, storing, transporting or shipping.
Must make available, free of charge at a reasonable time and place, to all employees at risk of exposure within 10 working days of initial assignment.