Biosafety Training. University of Ottawa * Office of Risk Management Human Resources - Occupational Health Disability & Leave. v0501. Biosafety Outline. Introduction Laboratory Associated Infections Blood-borne Pathogens Classification of Biohazards Infection/Biohazard Control
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University of Ottawa
*Office of Risk Management
Human Resources - Occupational Health Disability & Leave
A potential hazard to humans, animals or the environment caused by a biological organism, or by material produced by such an organism
Route of Transmission
Occupational Health, Disability and Leave Office telephone ext. 1472 http://www.uottawa.ca/services/hr/frames.html
Includes: - Education
- Hand washing
- Wearing protective barriers
- Use safe work practices
If samples cannot be guaranteed non-infective …… treat as infectious!
Class D, division 3 of WHMIS (Poisonous and Infectious Material - Biohazardous Infectious Material)
Unlikely to cause disease in healthy workers or animals
Rarely cause serious human or animal disease
May cause serious disease
Likely to cause very serious disease
Genetic Engineering = in vitro incorporation of genetic material from one cell into another
Human & non-human primate, and mycoplasma-containing cell lines
A detailed risk assessment should be undertaken when using a new cell line.
Practices and Procedures
Personal Protective Equipment
Effectiveness of control is based on the integrity of the containment.
Before using the cabinet:
After completion of work:
- Decontamination with formaldehyde gas (ORM)
- Certification (ORM)
Program based, information and methods to minimize risk of exposure.
Risk Assessment: Know your Agent
Training & Education
Signs & Labeling
Use a BSC for all operations with infectious material
Fill syringes carefully
Shield needles when withdrawing from stoppers
Do not bend, shear or recap needles.
Dispose of all used needles/syringes in yellow sharps containersNeedles and Syringes
Eye & Face Production
Items that CAN be autoclaved:
Items that CAN NOT be autoclaved:
Preparation of waste:
All users/operators must fill out the Autoclave User Questionnaire and receive training!
Vegetative bacteria(E.coli, Staph)
Mycobacteria and fungi
Spore forming bacteria(Bacillus)
Enveloped (HIV, Herpes)
Non enveloped (Hepatitis, Adenovirus)
Discarded biological material from teaching, clinical and research laboratories and operations. Biomedical waste includes but is not limited to;
Biomedical Waste (untreated)
Biomedical Waste (treated)
In compliance with Sewer use by-laws
With H2O 1:10