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Post-Processing Interventions to Control Listeriosis

Post-Processing Interventions to Control Listeriosis. Don L. Zink, Ph.D. Office of Plant and Dairy Foods and Beverages Center for Food Safety and Applied Nutrition Food and Drug Administration. Risk Management Strategies.

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Post-Processing Interventions to Control Listeriosis

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  1. Post-Processing Interventions to ControlListeriosis Don L. Zink, Ph.D. Office of Plant and Dairy Foods and Beverages Center for Food Safety and Applied Nutrition Food and Drug Administration

  2. Risk Management Strategies • Eliminate L. monocytogenes from the environment of processing plants that produce ready-to-eat foods • Use a pasteurization processes to destroy L. monocytogenes • In-pack pasteurization, or • Immediately before packaging • Use product formulations that prevent the growth of L. monocytogenes

  3. Steam Surface Pasteurization* * A joint development by ALKAR-RapidPak, Inc., Kraft/Oscar-Mayer and USDA-ERRC SSP module extends length by two indexes • Provides a surface kill step immediately before packaging • Adapts to existing line, no effect on line speed or packaging cost Graphics and information provided by ALKAR-RapidPak, Inc.

  4. Steam Surface Pasteurization Machine Configuration Film Travel Direction Top film Discharge Cut Seal Cool SSP Load Form Preheat heat treatment SSP step = 1.5 sec of high pressure steam Graphics and information provided by ALKAR-RapidPak, Inc.

  5. Steam Surface Pasteurization Vacuum Cooling Steam Injection Servo lifts product pedestal up into SSP chamber Graphics and information provided by ALKAR-RapidPak, Inc.

  6. Steam Surface Pasteurization High Pressure Steam Injection Vent Condensate Ports • Maximum contact time on most difficult area --- ends • 1.5 second cycle time with 4 alternating steam bursts per cycle Bursts 1 and 3 Bursts 2 and 4 Graphics and information provided by ALKAR-RapidPak, Inc.

  7. Steam Surface Pasteurization • Hot dogs inoculated with indicator microorganism • SSP treatment = 1.5 seconds • 4-log reduction for single-layer package configuration • Actual Listeria monocytogenes inoculated-pack tests (102 per package) resulted in zero positives • Double-layer package tests in progress • Preliminary findings show 2.0 - 2.5 log reduction Graphics and information provided by ALKAR-RapidPak, Inc.

  8. High Pressure Processing Data and images supplied by Dr. Peter Slade, NCFST

  9. High Pressure Processing • Uses the combined effects of temperature and high pressure to kill bacteria • Minimizes thermal damage to products • Reduces treatment time • Lethal effect of treatment can be modeled and characterized for each product type • Process can be applied to packaged product • Not limited to surface-only effects, thus is applicable to sliced products • A Batch process with significant capital costs Data and images supplied by Dr. Peter Slade, NCFST

  10. High Pressure Processing t = 4.0 minutes -0.8 -2.5 -4.2 -5.8 Log Reduction (N/N0) -7.5 43.00 48.50 54.00 30.00 Pressure (MPa) 35.00 59.50 40.00 Data and images supplied by Dr. Peter Slade, NCFST 45.00 50.00 65.00 Temperature °C

  11. High Pressure Processing P = 65 Kpsig (450 MPa) T = 40°C t = 6.0 minutes Data and images supplied by Dr. Peter Slade, NCFST

  12. In-Package Pasteurization Stork RMS-Protecon (Townsend) Steam-Based Post-Process Pasteurization System Data and images supplied by Drs. James Marsden and Randall Phebus, Kansas State University

  13. In Package Pasteurization Log cycle reduction of Listeria monocytogenes at 96.1°C Data and images supplied by Drs. James Marsden and Randall Phebus, Kansas State University

  14. Product Formulation Sodium or Potassium Lactate • Weak acid. • Disrupts membrane pH gradients • Inhibit energy metabolism. • Lactate alone only bacteriostatic at high concentrations. Sodium Diacetate • Dissociates into acetic acid and sodium acetate. • Lowers pH due to presence of acetic acid • Sodium diacetate alone only bacteriostatic at high levels. Data and images supplied by Dr. Paul Hall, Kraft Foods

  15. Predicted Growth Rate (wk-1) 0.05 0.04 0.03 0.02 0.01 75 71 67 0 63 59 2.5 Product Moisture (%) 3.5 4.5 55 5.5 6.5 7.5 Potassium Lactate Syrup (%) Product Formulation Influence of Lactate and Moisture on Predicted Growth Rate of L. monocytogenes in a Cured RTE Meat Salt = 2.2 %, Diacetate = 0.1% Data and images supplied by Dr. Paul Hall, Kraft Foods

  16. Product Formulation Influence of Lactate and Moisture on Predicted Growth Rate of L. monocytogenes in an Uncured RTE Meat Salt = 2.2 %, Diacetate = 0.1% 0.24 0.2 0.16 Predicted Growth Rate (wk-1) 0.12 0.08 0.04 75 71 67 0 63 2.5 3.5 59 4.5 5.5 6.5 Product Moisture (%) 55 7.5 Potassium Lactate Syrup (%) Data and images supplied by Dr. Paul Hall, Kraft Foods

  17. Product Formulation Application of Lactate – Diacetate to Cured RTE Meat Products • The model has been incorporated into a spreadsheet. • Composition (moisture, salt, lactate and diacetate) and an assumption about inoculum are input. • Growth parameters and a predicted growth curve are generated. • The model is used interactively to develop formulations that predict acceptable product quality and inhibit Listeria growth. Data and images supplied by Dr. Paul Hall, Kraft Foods

  18. Product Formulation Application of Lactate – Diacetate to Uncured RTE Meat Products • Growth rates in uncured products are much higher • Nitrite inhibits the growth of Listeria • Rigid environmental control programs are important for both cured and uncured products Data and images supplied by Dr. Paul Hall, Kraft Foods

  19. Product Formulation Data and images supplied by Dr. Paul Hall, Kraft Foods

  20. Product Formulation Inhibition of Growth of L. monocytogenes at 4°C by Buffered Sodium Citrate (IonalTM) Surface inoculum on beef franks Data and images supplied by Drs. James Marsden and Randall Phebus, Kansas State University

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