flow cytometry
Download
Skip this Video
Download Presentation
Flow cytometry

Loading in 2 Seconds...

play fullscreen
1 / 20

Flow cytometry - PowerPoint PPT Presentation


  • 667 Views
  • Uploaded on

Flow cytometry. Powerful technique for analysis of multiple parameters of individual cells within heterogeneous populations multiple physical characteristics of single particles or cells. What is flow cytometry?. scheme of flow cytometry. The fluidic system & optics system.

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about 'Flow cytometry' - Renfred


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
what is flow cytometry
Powerful technique for

analysis of multiple parameters of individual cells within heterogeneous populations

multiple physical characteristics of single particles or cells

What is flow cytometry?
the fluidic system optics system
The fluidic system & optics system
  • heart of the system
  • where the laser and the sample intersect and the optics collect the resulting scatter and fluorescence
the fluidic system
The fluidic system
  • Particles or cells are passed through the laser beam one at a time

Laser

Hydrodynamic focusing

size comparison by flow cytometry
Size comparison by flow cytometry
  • ~ 1 ~ 15 uM size detection
forward scatter
Forward scatter
  • Light is quantified by a detector that converts intensity into voltage
  • Cell size
side scatter
Side scatter
  • Granularity and structural complexity inside the cell
compensation16
Compensation
  • No-stained sample
  • Only FITC stained sample
  • Only PE stained sample

uncompensated

compensated

PE

FITC

application apoptosis
Application (apoptosis)
  • Apoptosis (FSC/SSC)

Apoptosis

SSC

Necrosis

FSC

application apoptosis18
Application (apoptosis)
  • Apoptosis (Annexin V/PI assay)

Normal Cell

Apoptotic Cell

: Phosphatidyl Serine

: Annexin V-FITC

application apoptosis19
Application (apoptosis)
  • Apoptosis (Annexin V/PI assay)

Control

Anti-Fas Ab

Propidium Iodide (PI)

Annexin V-FITC

application cell cycle
Application (cell cycle)

Apoptotic DNA Histogram

A typical DNA Histogram

G0-G1

G2-M

S

Apoptotic cells

Normal

G0/G1 cells

# of Events

Fluorescence Intensity

ad