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BPEX AI meeting . State of the art in conventional AI on Danish farms ___ Mads Thor Madsen DVM, Certificate in Swine Herd Health 14th of February 2005. Agenda – BPEX AI-meeting. Welcome and presentation Rules for Danbred AI stations AI research projects – preliminary results

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Bpex ai meeting l.jpg

BPEX AI meeting

State of the art in conventional AI on Danish farms

___

Mads Thor Madsen

DVM, Certificate in Swine Herd Health

14th of February 2005


Agenda bpex ai meeting l.jpg

Agenda – BPEX AI-meeting

  • Welcome and presentation

  • Rules for Danbred AI stations

  • AI research projects – preliminary results

  • State of the art in conventional AI

  • Future co-operation


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Heat detection

Ovulation

Riding test

Strenght of heat

Riding test + 4 boars

Time

Mounting

No mounting

(mod.e. Langendijk et al.)


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Heat detection


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Heat detection


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Heat detection


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Heat detection


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Heat detection


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Average and interval of oestrus duration and time of ovulation after onset of heat


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Optimal insemination

Total born piglets per litter

Total born

n = 75

X = 12,8 (4-25)

Ref.: Anne Kirstine Nissen, 1995


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Optimal insemination


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Optimal insemination


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Optimal insemination


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Optimal insemination – heat detection scheme


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Human stimulation

Oxytocin - lab trial

Investigated with 3 groups of sows

  • high stimulation

  • low stimulation

  • mating with boar (control)

    Blood sampling and hormone analysis

    All sows had the possibility of nose contact with a boar


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Human stimulation


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Human stimulation

Oxytocin – Herd trial

Investigated with 2 groups of sows

  • high stimulation

  • low stimulation

    Farrowing rate and total born piglets per litter


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Human stimulation

Oxytocin – Herd trial

Farrowing rate – a difference of 5.9 % in favour

of the high stimulation group

No difference in total born piglets per litter


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Stress and implantation

  • Implantation defined as day 0 – 34 after first insemination

  • Stress in part of the implantation will result in loss of embryos or return to oestrus

  • When and what stressors - we do not know?


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Stress and implantation

  • Transport of embryos – high stress

  • Day 10 – 12 maternal contact – low stress

  • Day 12 – 14 embryo production of estrogens – low stress

  • Until day 16 – low immune response – low stress

  • Until day 25 – heat stress causes death of embryos


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Stress and implantation

Conclusion

A Danish average sow will be most sensitive

to stress day 10 – 25 of the implantation period


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Stress and implantation

  • Stress releases a hormone from the pituitary gland

  • The hormone consists of ACTH and beta-endofine

  • ACTH releases cortisol and adrenaline, while beta-endofine is the problem

  • Beta-endofine inhibits the hormone responsible for ovulation (LH)


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Stress and implantation

Direct stress – lab trial

Sows in 3 groups treated every 4 hours

during oestrus with

- CRH

- ACTH

- Saline

Blood sampling and analyses of hormones


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Stress and implantation

Direct stress – lab trial

- Delayed LH peak and a more broad peak

  • Delayed ovulation (8 hours)

  • Unaffected length of heat (60 hours)


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Stress and implantation

Ovulation

BPT

Delayedovulation

Oestrus

BPT + 4 boars

Mounting

No mounting

Time


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Stress and time after AI

Herd trial

Rest after AI in boxes in 2 groups of sows which

were

  • Within the box for at least 2 hours after AI

  • Forced out of the box immediately after AI

    Farrowing rate and total born piglets per litter


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Stress and time after AI

Herd trial

No difference overall between the 2 groups

in farrowing rate and total born piglets per litter

But age dependent !


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Stress and time after AI


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Stress and time after AI

Farrowing rate and age


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Stress and time after AI

Total born piglets per litter and age


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Strategic use of boar at stimulation

Herd trial

Investigated with 2 groups of sows:

- Constant boar contact

- Only boar contact the first 2 days after weaning and at the day of expected insemination

Farrowing rate and total born piglets per litter

Time for heat detection and insemination


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Strategic use of boar at stimulation

No differences in farrowing rate and total born

piglets per litter

Reduction of time for heat detection and

insemination (approx. 2 minutes) in favour of

the low boar exposed group


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Miscellaneous

  • Avoid blood on tip of catheter

  • Avoid the use of catheter gels

  • Avoid preheating of semen before use

  • Use a higher concentration of Yorkshire semen when used on Yorkshire sows

  • Use a shorter life-span when using Hampshire semen

  • Re-inseminate if peristaltic contractions (2 hours later)

  • Boars for smell has to be > 10 month of age


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Prostaglandin (PGF2alfa) release during AI

PROSTAGLANDIN – TRIAL

Investigated with 3 groups of sows

  • high stimulation

  • low stimulation

  • boar stimulation

    Blood sampling and analyses of hormones


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Prostaglandin (PFG2alfa) release during AI


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