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BPEX AI meeting . State of the art in conventional AI on Danish farms ___ Mads Thor Madsen DVM, Certificate in Swine Herd Health 14th of February 2005. Agenda – BPEX AI-meeting. Welcome and presentation Rules for Danbred AI stations AI research projects – preliminary results

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Bpex ai meeting l.jpg
BPEX AI meeting

State of the art in conventional AI on Danish farms

___

Mads Thor Madsen

DVM, Certificate in Swine Herd Health

14th of February 2005


Agenda bpex ai meeting l.jpg
Agenda – BPEX AI-meeting

  • Welcome and presentation

  • Rules for Danbred AI stations

  • AI research projects – preliminary results

  • State of the art in conventional AI

  • Future co-operation


Heat detection l.jpg
Heat detection

Ovulation

Riding test

Strenght of heat

Riding test + 4 boars

Time

Mounting

No mounting

(mod.e. Langendijk et al.)








Optimal insemination l.jpg
Optimal insemination ovulation after onset of heat

Total born piglets per litter

Total born

n = 75

X = 12,8 (4-25)

Ref.: Anne Kirstine Nissen, 1995


Optimal insemination11 l.jpg
Optimal insemination ovulation after onset of heat


Optimal insemination12 l.jpg
Optimal insemination ovulation after onset of heat


Optimal insemination13 l.jpg
Optimal insemination ovulation after onset of heat


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Optimal insemination – heat detection scheme ovulation after onset of heat


Human stimulation l.jpg
Human stimulation ovulation after onset of heat

Oxytocin - lab trial

Investigated with 3 groups of sows

  • high stimulation

  • low stimulation

  • mating with boar (control)

    Blood sampling and hormone analysis

    All sows had the possibility of nose contact with a boar


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Human stimulation ovulation after onset of heat


Human stimulation17 l.jpg
Human stimulation ovulation after onset of heat

Oxytocin – Herd trial

Investigated with 2 groups of sows

  • high stimulation

  • low stimulation

    Farrowing rate and total born piglets per litter


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Human stimulation ovulation after onset of heat

Oxytocin – Herd trial

Farrowing rate – a difference of 5.9 % in favour

of the high stimulation group

No difference in total born piglets per litter


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Stress and implantation ovulation after onset of heat

  • Implantation defined as day 0 – 34 after first insemination

  • Stress in part of the implantation will result in loss of embryos or return to oestrus

  • When and what stressors - we do not know?


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Stress and implantation ovulation after onset of heat

  • Transport of embryos – high stress

  • Day 10 – 12 maternal contact – low stress

  • Day 12 – 14 embryo production of estrogens – low stress

  • Until day 16 – low immune response – low stress

  • Until day 25 – heat stress causes death of embryos


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Stress and implantation ovulation after onset of heat

Conclusion

A Danish average sow will be most sensitive

to stress day 10 – 25 of the implantation period


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Stress and implantation ovulation after onset of heat

  • Stress releases a hormone from the pituitary gland

  • The hormone consists of ACTH and beta-endofine

  • ACTH releases cortisol and adrenaline, while beta-endofine is the problem

  • Beta-endofine inhibits the hormone responsible for ovulation (LH)


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Stress and implantation ovulation after onset of heat

Direct stress – lab trial

Sows in 3 groups treated every 4 hours

during oestrus with

- CRH

- ACTH

- Saline

Blood sampling and analyses of hormones


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Stress and implantation ovulation after onset of heat

Direct stress – lab trial

- Delayed LH peak and a more broad peak

  • Delayed ovulation (8 hours)

  • Unaffected length of heat (60 hours)


Stress and implantation25 l.jpg
Stress and implantation ovulation after onset of heat

Ovulation

BPT

Delayedovulation

Oestrus

BPT + 4 boars

Mounting

No mounting

Time


Stress and time after ai l.jpg
Stress and time after AI ovulation after onset of heat

Herd trial

Rest after AI in boxes in 2 groups of sows which

were

  • Within the box for at least 2 hours after AI

  • Forced out of the box immediately after AI

    Farrowing rate and total born piglets per litter


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Stress and time after AI ovulation after onset of heat

Herd trial

No difference overall between the 2 groups

in farrowing rate and total born piglets per litter

But age dependent !


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Stress and time after AI ovulation after onset of heat


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Stress and time after AI ovulation after onset of heat

Farrowing rate and age


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Stress and time after AI ovulation after onset of heat

Total born piglets per litter and age


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Strategic use of boar at stimulation ovulation after onset of heat

Herd trial

Investigated with 2 groups of sows:

- Constant boar contact

- Only boar contact the first 2 days after weaning and at the day of expected insemination

Farrowing rate and total born piglets per litter

Time for heat detection and insemination


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Strategic use of boar at stimulation ovulation after onset of heat

No differences in farrowing rate and total born

piglets per litter

Reduction of time for heat detection and

insemination (approx. 2 minutes) in favour of

the low boar exposed group


Miscellaneous l.jpg
Miscellaneous ovulation after onset of heat

  • Avoid blood on tip of catheter

  • Avoid the use of catheter gels

  • Avoid preheating of semen before use

  • Use a higher concentration of Yorkshire semen when used on Yorkshire sows

  • Use a shorter life-span when using Hampshire semen

  • Re-inseminate if peristaltic contractions (2 hours later)

  • Boars for smell has to be > 10 month of age


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Prostaglandin (PGF2alfa) release during AI ovulation after onset of heat

PROSTAGLANDIN – TRIAL

Investigated with 3 groups of sows

  • high stimulation

  • low stimulation

  • boar stimulation

    Blood sampling and analyses of hormones


Prostaglandin pfg2alfa release during ai l.jpg
Prostaglandin (PFG2alfa) release during AI ovulation after onset of heat


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