Lab Exercise 0ne. Carbohydrate Analysis Lab A.1 Page 26. Biochemical Assay. Biochemistry deals with the identification and quantification of bio-molecules from a variety of living systems
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We will exploit its ability to reduce other compounds to produce a product which can be measured optically
hemiacetal and open chain
is driven to open chain as
oxidation to acid form takes
place. This ensures a
quantitative conversion with
time and a stoicheometric
production of reduced copper.
The following graph shows standard curve of glucose concentration. Absorbency readings were taken at 540 nanometers of 5 samples with known glucose concentration. R2 value of .9688 indicates a fit linear correlation. The slope of this graph was used to calculate glucose concentration in unknown samples (Fig 4).
Fig 3: Graph of concentration of “standard” glucose vs. absorbancy at 540 nm for tubes 1-5.