Hong Kong Association of Blood Transfusion & Haematology NAT and Viral Safety in Blood Transfusion Dr. P.H. Yu Medical Officer Department of Pathology Tseung Kwan O Hospital Viral Safety in Blood Transfusion
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Dr. P.H. Yu
Department of Pathology
Tseung Kwan O Hospital
Adapted from Transfusion 2000; 40:143-159
Source: (1) Muller-Breitkreutz K for the EPFA Working Group on Quality Assurance. Results of viral marker screening of unpaid donations and probability of window donations in 1997. Vox Sang 2000;78:149-157 (2) Aubuchon JP, Birkmeyer JD, Busch MP. Safety of the blood supply in the United States: opportunites and controversies. Ann Int Med 1997;127:904-909. (3) Regan FAM, Hewitt P, Barabara JAJ, Contreras M.on behalf of the current TTI Study Group Prospective investigation of transfusion in transmitted infection in recipients of over 20000 units of blood, Br Med J 2000;320:403-406. (4) Tosti ME, et al, An estimate of the current risk of transmitting blood-borne infections through blood transfusion in Itly. Br J Haemat, 2002;117:215-219.
1)Sample preparation & target capture
1)Sample preparation & target captureRNA hybridized to target-specific oligonucleotides and then captured onto magnetic microparticles which are separated from plasma in a magnetic field
2)Transcription Mediated Amplification - single-step isothermal amplification - initial synthesis of cDNA from the target RNA followed by in-vitro transcription of cDNA into many copies of RNA amplicon
3) Detection by a chemiluminescent probe which hybridized to the amplicon
The system includes a robotic pipettor (Tecan; Durham, NC), the Chiron Procleix target capture system, and the Procleix Leader HC+ with the Procleix system software.
4) Hybridization of products to oligonucleotide peroxidase conjugated probe
5) Detection of probe-bound products by colorimetric determination
HIV the Chiron Procleix target capture system, and the Procleix Leader HC+ with the Procleix system software.
Yield of NAT Screening on Blood Donors infectious agents transmitted by blood by NAT