Producing large beads from alginate
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Producing large beads from alginate. Principle and equipment. Principle. Alginate solution + divalent cations (Ca, Ba, etc.) = solidified matrix (gel-like material). If alginate solution is forced into a droplet shape, the result will be a solid bead (sphere).

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Producing large beads from alginate l.jpg

Producing large beads from alginate

Principle and equipment


Principle l.jpg
Principle

Alginate solution +divalent cations

(Ca, Ba, etc.)=solidified matrix(gel-like material)

If alginate solution is forced into a

droplet shape, the result will be a

solid bead (sphere)


Producing beads in small quantities l.jpg
Producing beads in small quantities

  • The alginate solution is dripped, one drop at the time from a large syringe into solution of calcium chloride

  • The beads are instantly formed in the CaCl2 solution



If all you need are a few grams of beads for your experiments l.jpg

…If all you need are a few grams of beads for your experiments…

The method just shown is ideal and you need not read further.

However…….


If your experiment requires large quantities of beads one or more liters l.jpg

…If your experiment requires large quantities of beads (one or more liters)…

You will find the syringe method very tiresome, especially when hard beads are needed when employing highly viscous alginate solutions

Some equipment is needed


Equipment for producing large beads 2 4 mm dia l.jpg
Equipment for producing large beads (2-4 mm dia.) (one or more liters)…

  • This is essentially a showerhead metal container,

  • With specially-designed nozzles.

  • Bead form by the force of gravity.


In real life the device looks like this l.jpg
In real life, the device looks like this. (one or more liters)…

Alginate solution

Droplets

Nozzle plate

Formed beads


It can be made in a smaller size or larger l.jpg
It can be made in a smaller size (or larger). (one or more liters)…

Nozzle plate


Slide10 l.jpg
Immobilization (entrapment) of one bacterial species and co-immobilization of two (or more) microorganisms inside the bead

Chlorella

+

Azospirillum

+ Alginate

= co-immobilization

3 mm


Potential use of large beads l.jpg
Potential use of large beads co-immobilization of two (or more) microorganisms inside the bead

  • Industry (production of microbial compounds)

  • Wastewater treatment (removal of contaminants)

  • Agriculture (production of bacterial inoculants)


Industry fermentation l.jpg
Industry (fermentation) co-immobilization of two (or more) microorganisms inside the bead

Microorganisms entrapped in alginate are

often grown in large fermentation vats,

similar to the vat pictured at left.

The products they produce are harvested,

while the beads remain inside the vat.


Wastewater treatment l.jpg
Wastewater treatment co-immobilization of two (or more) microorganisms inside the bead

Something for the future--

Large-scale treatment by

immobilized and co-

immobilized microorganisms


Agriculture bacterial inoculants before sowing l.jpg
Agriculture (bacterial inoculants) before sowing co-immobilization of two (or more) microorganisms inside the bead

Beads

Seeds


Agriculture dry alginate beads sowed in a wheat field l.jpg
Agriculture (dry alginate beads sowed in a wheat field) co-immobilization of two (or more) microorganisms inside the bead

BEADS

SEEDS


Other applications l.jpg

Other applications? co-immobilization of two (or more) microorganisms inside the bead

The sky is the limit…

Use your imagination


Some unexpected benefits l.jpg
Some unexpected benefits co-immobilization of two (or more) microorganisms inside the bead

  • It does not require human energy, let alone paid energy.

  • It is accepted technology, published in most scientific journals. (Your “brilliant” paper will not be rejected on the basis of an untried method.)

  • As an environmentally-friendly technology, using it will make you look good.

  • Have fun…..


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