Ahmed Mohamed Marzouqi Hasan Salem Yalyali Ali Mousa Al Masri Essa Al Muallemi. Algae Based Biodiesel. Introduction. Goals: produce biodiesel from microalgae Saving the environment from CO 2 emissions Using sewage water Produce a renewable and new energy source
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Sub culturing the algae in bottles
Cultivating micro algae in photobioreactor
Cultivating microalgae using diesel engine emissions
Separating algae cells from water by:
Cultivating microalgae in sewage water
Crushing algae cells by:
Doing Transteterification to get biodiesel
Extracting oil from algae cells by hexane
Testing the biodieselProject Overview
Soil water(not for Nannochloropesis) 400 ml/L
NaCl 100 ml/L
NaNO3 300 ml/L
K2HPO4 100 ml/L
CaCl 100 ml/L
KH2PO4 100 ml/L
MgSO4 100 ml/L
Vitamins 10 ml/L for each
P (4) metal solution 60 ml/L
10 LDaily Media Preparation
Four Rooms (40 X 40 X 75 cm)
Offset to install the neon lights (5 cm) from all sides
The outer case of the cultivation reactor is made of 6mm thickness plastic with 10mm base. The offset will be filled with neon lights. The amount of light supported to the culture has a proportional effect with the algae growth rate.
The bottom case of the reactor has been drilled to feed the culture with CO2 and to drain the water if needed. Water and nutrients will be fed from the upper cover of the room. CO2 are being used in cylinders, and it is connected to the reactor with tubes which will bubble the CO2 in the reactor, and the bubbling will help the media to be mixed at the same time.
The base of the reactor has been manufactured to keep the reactor with a suitable height from the ground and to be moved easily with four wheels from its bottom, with considering the high volume of the cultivation reactor, which will lead for a high mass of it, the base has been manufactured with a thick iron material to avoid any fracture or cracks.
Once algae has been ordered from University of Texas, the manual of the media that will help algae growing efficiently was provided, where the media name is Bristol, which consists of many chemical components and Proteose Peptone with 6.8 pH. The components of Bristol medium are:
A bioreactor which has a shape near to the cultivation reactor will be used to grow microalgae strain with feeding it from the diesel engine's exhaust gases.
Design specifications: reactor with a suitable height from the ground and to be moved easily with four wheels from its bottom, with considering the high volume of the cultivation reactor, which will lead for a high mass of it, the base has been manufactured with a thick iron material to avoid any fracture or cracks.
The leakage problem occurred for the cultivation reactor happened for the bioreactor also. Therefore, it was covered by double plastic layer to prevent the leakage.
There are many methods for determining total organic carbon (TOC). TOC analyzer is one of the most important analytical methods to know how much the TOC in the sample. Four samples have been taken to test their TOC using the TOC analyzer in the civil engineering department. These samples are:
The steps of using the device: reactor with a suitable height from the ground and to be moved easily with four wheels from its bottom, with considering the high volume of the cultivation reactor, which will lead for a high mass of it, the base has been manufactured with a thick iron material to avoid any fracture or cracks.
-burn it at 800 c.
It is observed that while the output of the diesel engine increases, the TOC will increase.
Filtration is carried out commonly on membranes of modified Cellulose with the aid of a suction pump.
In this method the algae are kept in low height containers under the sun light.
Flocculation is a method of separating algae from the medium by using chemicals to force the algae to form lumps.
Usually flotation is used in combination with flocculation for Algae Harvesting in waste water. It is a simple method by which algae can be made to float on the surface of the medium and removed as scum.
Centrifugation is a method of separating algae from the medium by using a centrifuge to cause the algae to settle to the bottom of a flask or tank.
Three methods were used in the project to separate microalgae from water: -
= 1.547 m3 x 1000 AED/ m3 = 1547 AED