Open Field Testing of Rodents
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Open Field Testing of Rodents. Anxiety – Gross Motor Behavior - Open Field Test . Observe species-common (behavior: rearing sniffing, grooming) Observe thigmotaxic behavior (stay at walls). 48 cm. Place animal in a corner square for a designated amount of time

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Anxiety – Gross Motor Behavior - Open Field Test

Observe species-common

(behavior: rearing

sniffing, grooming)

Observe thigmotaxic

behavior (stay at walls)

48 cm

Place animal in a corner square for a designated amount of time

then observe the number of squares transversed, rearing, defecation etc.

 Anxiolytics serve to increase exploratory behavior


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  • 5 min test

  • DVs – What type of data do we have???? Nominal, Scale, Ratio???

  • Latency (in sec)

  • Squares Transversed (#)

  • Rearing (#)

  • Asst. Rearing (#)

  • Grooming (#)

  • Fecal Boli (#)

  • Urination (#)

  • Thigmotaxic behavior

* Already Taken (no more than 13.5 units)

* Already Taken (no more than 13.5 units)


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  • IVs???

  • Environment

    Standard, Enriched

  • Gender

    Male, Female

    What is the Design Notation?

2X2


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In your Method you need:

Subjects???

Who are they? (age, gender)

Where did you get them? (vendor)

How are they housed?

What is their L/D cycle?

Protocol Approval?

Apparatus???

What equipment will you be using????

(Model#, City etc…)

Open Field Specifics

Any other equipment

Procedure???

Take notes!!!!


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What are your hypotheses?

Can have more than one…

Rats are nocturnal…

Male vs Female…

Older Rats….


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