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Open Field Testing of Rodents

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Open Field Testing of Rodents. Anxiety – Gross Motor Behavior - Open Field Test . Observe species-common (behavior: rearing sniffing, grooming) Observe thigmotaxic behavior (stay at walls). 48 cm. Place animal in a corner square for a designated amount of time

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slide2

Anxiety – Gross Motor Behavior - Open Field Test

Observe species-common

(behavior: rearing

sniffing, grooming)

Observe thigmotaxic

behavior (stay at walls)

48 cm

Place animal in a corner square for a designated amount of time

then observe the number of squares transversed, rearing, defecation etc.

 Anxiolytics serve to increase exploratory behavior

slide3

5 min test

  • DVs – What type of data do we have???? Nominal, Scale, Ratio???
  • Latency (in sec)
  • Squares Transversed (#)
  • Rearing (#)
  • Asst. Rearing (#)
  • Grooming (#)
  • Fecal Boli (#)
  • Urination (#)
  • Thigmotaxic behavior

* Already Taken (no more than 13.5 units)

* Already Taken (no more than 13.5 units)

slide4

IVs???

  • Environment

Standard, Enriched

  • Gender

Male, Female

What is the Design Notation?

2X2

slide5

In your Method you need:

Subjects???

Who are they? (age, gender)

Where did you get them? (vendor)

How are they housed?

What is their L/D cycle?

Protocol Approval?

Apparatus???

What equipment will you be using????

(Model#, City etc…)

Open Field Specifics

Any other equipment

Procedure???

Take notes!!!!

slide6

What are your hypotheses?

Can have more than one…

Rats are nocturnal…

Male vs Female…

Older Rats….

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