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DNA – The building blocks of life. DNA – The building blocks of life. DNA stands for Deoxyribonucleic Acid and is responsible for: a) storing and passing on genetic information from one generation to another. b) Provides the instructions for creating proteins.

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dna the building blocks of life2
DNA– The building blocks of life

DNA stands for Deoxyribonucleic Acid and is responsible for:

a) storing and passing on genetic information from one generation to another.

b) Provides the instructions for creating proteins.

slide3
The DNA structure is shaped like a spiral staircase, or twisted ladder.

The outer parts of the ladder are made of sugars called deoxyribose which are connected by phosphate groups.

The rungs of the ladder are made of pairs of nitrogen bases held together by hydrogen bonds.

a c c g t t t t g a c a c a t g g c a a a a c t g t g t
There are four possible DNA bases

Adenine – A

Guanine – G

Thymine – T

Cytosine - C

There is only one way these bases can bond together.

A C C G T T T T G A C A C AT G G C A A A A C T G T G T
  • Purines
  • have a double ring base
  • Pyrimidines
  • - have a singe ring base

Adenine pairs with Thymine Guanine pairs with Cytosine.

slide5
It is the order of these nitrogen bases that produces the genes for a trait.

The specific sequence of the nitrogen bases are instructions (or a code) for making proteins which in turn present as a particular trait.

Because there can be so many different combinations of the bases, the code can be limitless.

dna replication
DNA Replication

Replication is the process where DNA molecules form exact duplicates.

Replication is essential for cellular division so that new daughter cells will have a complete copy of the genetic information.

dna replication7
DNA Replication

Simply stated:

1. The ladder splits

2. Free floating nitrogen bases pair with the opened strand

3. Two new DNA strands are formed.

now more details
Now more details

With the help of the helicase enzyme, the double helix is rotated and uncoiled.

The bonds between the complementary bases are then broken, splitting the double helix up the middle – like a zipper unzipping starting at one end and moving towards the other producing a Replication Fork

slide9
This exposes the two stands as single bases needing complements.

These 2 single strands

act as a template for

constructing a new

double helix and are

held apart by single

strand binding proteins.

slide10
Replication can not begin with out an identified starting point. An enzyme called Primase is “tags” the DNA template to initiate the synthesis of the new DNA segment.
slide11
New nucleotides are brought from a pool of free nucleotides present in the cytoplasm to bind with the complimentary bases.

The new strand is assembed by the enzyme DNA polymerase which is guided along the chain by a sliding clamp.

DNA polymerase proof-reads the new strand

– 1 error in about

10 billion.

slide12
The RNA primers created to initiate replication are eventually removed by the enzyme RNase H leaving gaps between segments on the lagging strand. These segments are known as Okazaki fragments

Once replication is complete, DNA ligase join the segments together, sealing the double chain and reforming the helix.

dna replication animation tutorial
DNA Replication Animation Tutorial

http://www.wiley.com/college/pratt/0471393878/student/animations/dna_replication/

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