CHAPTER 6
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Rna

CHAPTER 6


Rna

RNA

  • RNADNARNA(transcription)RNARNA (tRNA, rRNA)

  • RNARNARNARNA (messenger RNA, mRNA)

CHAPTER 6


Rna

DNARNA(RNA polymerase) RNARNADNARNA

CHAPTER 6


Rna

CHAPTER 6


Rna

  • RNA (template strand)DNADNA (T) RNA (U) RNA (coding strand) DNARNADNA5-3-

CHAPTER 6


Rna

CHAPTER 6


Rna

  • (housekeeping genes)

CHAPTER 6


Rna

  • (cistron)(structural gene) DNA / RNADNA

  • (open reading frame, ORF) DNARNA

CHAPTER 6


Rna

  • mRNAmRNAmRNAmRNARNA (monocistronic mRNA)

CHAPTER 6


Rna

CHAPTER 6


Rna

  • ((operon)) mRNARNAmRNA (polycistronic mRNA)

CHAPTER 6


Rna

  • DNA(promoter)RNA5-(upstream region)

  • mRNAmRNA5-(5-untranslated region) (5-UTR)mRNA3- (3-untranslated region ) (3-UTR)

CHAPTER 6


Rna

CHAPTER 6


Rna

  • RNA(core enzyme) () sigma(sigma subunit)RNAsigmasigma ()DNADNA10(10 region)35(35 region)

CHAPTER 6


Rna

CHAPTER 6


Rna

  • sigmaRNADNA(transcription bubble)10TATAATATDNADNADNADNARNA

CHAPTER 6


Rna

CHAPTER 6


Rna

  • mRNAAG

  • RNA()

CHAPTER 6


Rna

CHAPTER 6


Rna

RNA

  • (terminator)DNA6(inverted repeats)

CHAPTER 6


Rna

CHAPTER 6


Rna

CHAPTER 6


Rna

  • Rho(Rho-dependent terminators) Rho(Rho (r) protein) RNADNARho(Rho-independent) ()Rho

CHAPTER 6


Rna

CHAPTER 6


Rna

CHAPTER 6


Rna

  • (constitutive gene) 1030

CHAPTER 6


Rna

  • 1035sigma(activator protein)

CHAPTER 6


Rna

CHAPTER 6


Rna

  • (transcription factor)

CHAPTER 6


Rna

CHAPTER 6


Rna

  • (negative regulation) (positive regulation) DNA

  • (negative regulation) (repressor)DNADNA(operator sequence)DNADNADNA

CHAPTER 6


Rna

CHAPTER 6


Rna

  • (signal molecule)

  • DNADNADNA (allosteric protein)

CHAPTER 6


Rna

CHAPTER 6


Rna

CHAPTER 6


Rna

  • RNA

  • RNA I (RNA polymerase I) RNA (rRNA) RNA III (RNA polymerase III) RNA (tRNA)5SRNA (5S rRNA)RNARNAII (RNA polymerase II)

  • (transcription factor)RNA

CHAPTER 6


Rrna trna

rRNAtRNA

  • rRNA18S28S rRNARNA (45S RNA)rRNArRNARNA I

CHAPTER 6


Rna

CHAPTER 6


Rna

  • rRNA (nucleolus) rRNA18S28S rRNArRNA(nucleolar organizer)rRNA

CHAPTER 6


Rna

CHAPTER 6


Rna

CHAPTER 6


Rna

CHAPTER 6


Rna

  • DNADNA(enhancer)

CHAPTER 6


Rna

  • RNA IIinitiator boxTATA box(upstream element)initiator boxDNA

  • TATA box / initiator box25TATA boxRNA IIIITBP (TATA (TATA binding protein) TATA box (TATA box factor))

CHAPTER 6


Rna

CHAPTER 6


Rna

  • TBPRNA IIIIIITBPTFIIDTFIIDRNA IITFIIATFIIBRNA IITFIIFTFIIFRNA IIRNA

CHAPTER 6


Rna

CHAPTER 6


Rna

CHAPTER 6


Rna ii

RNA II

  • TFII

CHAPTER 6


Rna

  • TFIIDTFIIBTFIIARNA IITFIID

  • GC boxCAAT boxAP1 (Octamer)

CHAPTER 6


Rna

  • DNARNA

CHAPTER 6


Rna

CHAPTER 6


Rna

  • DNA

CHAPTER 6


Rna

CHAPTER 6


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