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Antiviral treatment of calves persistently infected with bovine viral diarrhea virus

Antiviral treatment of calves persistently infected with bovine viral diarrhea virus. Benjamin Newcomer, DVM Auburn University January 26, 2009. Congenital defects. Previous Research. 1 previous study in PI calves Peek, et al, AJVR 2004 Evaluation of recombinant human interferon alfa-2a

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Antiviral treatment of calves persistently infected with bovine viral diarrhea virus

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  1. Antiviral treatment of calves persistently infected with bovine viral diarrhea virus Benjamin Newcomer, DVM Auburn University January 26, 2009

  2. Congenital defects Previous Research • 1 previous study in PI calves • Peek, et al, AJVR 2004 • Evaluation of recombinant human interferon alfa-2a • No antiviral activity • Microcytic anemia

  3. Antiviral compound DB772 • 2-(2-benzimidazolyl)-5-[4-(2-imidazolino)phenyl]furan dihydrochloride

  4. Study aim The aim of this study was to test the in vivo efficacy of a novel antiviral compound (DB772) in the treatment of PI calves.

  5. Methods • 6 PI calves used in study • 4 calves treated with DB772 q8hr x 6d • 2 calves treated with diluent only • Samples collected for virus isolation, viral titration and qPCR

  6. Results – qPCR of serum samples

  7. Results – qPCR of serum samples

  8. Results - viral titration of nasal swab samples

  9. Results - viral titration of nasal swab samples

  10. Results – virus isolation from WBC passages Calf A BVDV I Treated Calf B BVDV II Treated Calf C Calf D BVDV I Control Calf E BVDV II Control Calf F Day 0 1 3 5 7 14 21 = Positive = Negative

  11. Results – virus isolation from WBC passages Calf A BVDV I Treated Calf B BVDV II Treated Calf C Calf D BVDV I Control Calf E BVDV II Control Calf F Day 0 1 3 5 7 14 21 = Positive = Negative

  12. Results – virus isolation from WBC passages Calf A BVDV I Treated Calf B BVDV II Treated Calf C Calf D BVDV I Control Calf E BVDV II Control Calf F Day 0 1 3 5 7 14 21 = Positive = Negative

  13. Results – virus isolation from WBC passages Calf A BVDV I Treated Calf B BVDV II Treated Calf C Calf D BVDV I Control Calf E BVDV II Control Calf F Day 0 1 3 5 7 14 21 = Positive = Negative

  14. Results – virus isolation from WBC passages Calf A BVDV I Treated Calf B BVDV II Treated Calf C Calf D BVDV I Control Calf E BVDV II Control Calf F Day 0 1 3 5 7 14 21 = Positive = Negative

  15. Results – virus isolation from WBC passages Calf A * * BVDV I Treated Calf B * * * * BVDV II Treated Calf C * * Calf D BVDV I Control * Calf E BVDV II Control * Calf F Day 0 1 3 5 7 14 21 = Positive = Negative

  16. Susceptibility or Resistance of BVDV strains to 4 M concentration of DB772 when tested in vitro

  17. Susceptibility or Resistance of BVDV strains to 4 M concentration of DB772 when tested in vitro Day 14 Day 5 Day 7 Day 3

  18. Conclusions • Promising results early • Treatment of BVDV I seems more rewarding than BVDV II • Additional research is needed • Sequencing of viral isolates • Prevention of acute infection

  19. Thanks! • Dr. Dan Givens • Dr. Shonda Marley • Ms. Pat Galik • Mr. Drew Eason • Ms. Yijing Zhang • Dr. Kay Riddell • Dr. Paul Walz • Dr. Dave Boykin • Dr. Chris Dykstra

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