Tim Pengampu. Prof. Sebastian MarginoProf. Andi TrisyonoDr. TaryonoDr. Donny Widianto. Jadual Kuliah - PowerPoint PPT Presentation
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1. Bioteknologi Pertanian Pengantar
2. Tim Pengampu Prof. Sebastian Margino
Prof. Andi Trisyono
Dr. Donny Widianto
3. Jadual Kuliah & SAP
4. Jadual Kuliah & SAP
5. Buku Acuan Yuwono, T. 2006. Bioteknologi Pertanian. Gadjah Mada Press.
Glick, B.R. and J.J. Pasternak. 1994. Molecular Biotechnology: Principles and application of recombinant DNA. American Society for Microbiology, Washington, DC.
Watson, J.D., M. Gilman, J. Witkowski, and M. Zoller. 1992. Recombinant DNA. Scientific American Books, New York, USA.
Mantell, S.H., J.A. Matthews, and R.A. McKee. 1985. Principles of Plant Biotechnology: an introduction to genetic engineering in plants. Blackwell Scientific Publications, Oxford, UK.
Brown, C.M., I. Campbell, and F.G. Friest. 1987. Introduction to Biotechnology. Blackwell Scientific Publications, Oxford, UK.
Smith, J.E. 1985. Biotechnology Principles. Van Nostrand Reinhold Co Ltd., Berkshire, UK.
Drlica, K. 1984. Understanding DNA and Gene Cloning. John Wiley & Sons Inc., New York, USA.
6. Penilaian Total Nilai Ujian + Mid + Tugas dari 4 dosen dibagi 4
(Rata-rata dari Nilai Dosen I + II + III + IV)
A : > rata-rata kelas + 1,5 x stdev
B : < rata-rata kelas + 1,5 x stdev &
> rata-rata kelas + 0,5 x stdev
C : < rata-rata kelas + 0,5 x stdev &
> rata-rata kelas – 0,5 x stdev
D : < rata-rata kelas – 0,5 x stdev &
> rata-rata kelas – 1,5 x stdev
E : < rata-rata kelas – 1,5 x stdev
7. Tata Tertib Tepat waktu, toleransi maks. 15 menit
HP tidak diaktifkan
Hadir minimal 70%
Paham bahasa Indonesia & Inggris
Baca salah satu / dua buku acuan
Kerjakan Tugas, Mid, & Ujian
8. Tujuan Perkuliahan Mengenalkan dan memahamkan bahasa bioteknologi : Kosakata (istilah dalam bioteknologi), tatabahasa (Ilmu & Teknologi pendukung bioteknologi), struktur kalimat (Perakitan jasad rekombinan) dan arti (Manfaat, bioetika)
9. What is Biotechnology ?
10. mouse with human ear
11. Biological production
12. Food & Medicine production
13. Industrial Fermentation
14. What is Biotechnology ? using scientific methods with organisms to produce new products or new forms of organisms
any technique that uses living organisms or substances from those organisms to make or modify a product, to improve plants or animals, or to develop micro-organisms for specific uses
15. manipulation of genes is called genetic engineering or recombinant DNA technology
genetic engineering involves taking one or more genes from a location in one organism and either
Transferring them to another organism
Putting them back into the original organism in different combinations
What is Biotechnology ?
16. Related Knowledge & Industry
17. Goals of Biotechnology To understand more about the processes of inheritance and gene expression
To provide better understanding & treatment of various diseases, particularly genetic disorders
To generate economic benefits, including improved plants and animals for agriculture and efficient production of valuable biological molecules
18. Ancient biotechnology- early history as related to food and shelter; Includes domestication
Classical biotechnology- built on ancient biotechnology; Fermentation promoted food production, and medicine
Modern biotechnology- manipulates genetic information in organism; Genetic engineering
19. History of Domestication and Agriculture
Paleolithic peoples began to settle and develop agrarian societies about 10,000 years ago
Early farmers in the Near East cultivated wheat, barley, and possibly rye
7,000 years ago, pastoralists roamed the Sahara region of Africa with sheep, goats, cattle, and also hunted and used grinding stones in food preparation
Early farmers arrived in Egypt 6,000 years ago with cattle, sheep, goats, and crops such as barley, emmer, and chick-pea
Archaeologists have found ancient farming sites in the Americas, the Far East, and Europe ancient biotech
20. classical biotech Describes the development that fermentation has taken place from ancient times to the present
Top fermentation - developed first, yeast rise to top
1833 - Bottom fermentation - yeast remain on bottom
1886 – Brewing equipment made by E.C. Hansen and still used today
World War I – fermentation of organic solvents for explosives (glycerol)
World War II – bioreactor or fermenter:
Cholesterol – Steroids
21. old biotech meets new Fermentation and genetic engineering have been used in food production since the 1980s
Genetically engineered organisms are cultured in fermenters and are modified to produce large quantities of desirable enzymes, which are extracted and purified
Enzymes are used in the production of milk, cheese, beer, wine, candy, vitamins, and mineral supplements
Genetic engineering has been used to increase the amount and purity of enzymes, to improved an enzyme’s function, and to provide a more cost-efficient method to produce enzymes.
Chymosin, used in cheese production, was one of the first produced
22. first DNA cloning
23. Summary of biotechnology development
1750: B.C. Sumerians Brew Beer
1944: DNA confirmed as genetic material
1967: First protein synthesized by automation
1973: First recombinant DNA experiment by Cohen and Boyer
1973: Voluntary moratorium on certain cloning experiments
1974: Recombinant DNA Advisory Committee founded at NIH
1976: NIH Guidelines established
1980: Boyer Cohen patent issued (shared equally between Boyer, UCSF, Cohen, and Stanford)
1982: 1st animal vaccine made by DNA recombinant technology
1985: DNA Finger printing by Alec Jeffreys
24. Summary of biotechnology development 1987: First authorized field test of a GMO-Frostban, Adv. Genetic Sciences
1988: PCR developed by K. Mullis
1990: 1st Gene Therapy Trials in U.S.
1994: Flavr Savr Tamato- Calgene
1995: 1st DNA sequences of free-living organism completed
1996: Human Genome Project under way
1997:Dolly-First cloned mammal
2000: Human Genome sequence is complete 3 billion bases, 2 yrs ahead of schedule
2001: Copy Cat, Cloning pets for profit.
2002: First Human Clone?
2003: Dolly is put down, Final version of Human DNA Sequence published.
25. Organisms involved in biotechnology
26. Biotechnology in Agriculture
27. Drought-tolerance in APX-expressing plants
28. Insect Resistance
29. Resistance to Fungal Disease
30. Viral Resistance
31. Herbicide Resistance
32. Modification of Cotton Fiber Length
33. Biotechnology in Health
34. Vitamin fortified staple food
35. Edible Vaccines – A Biopharming
36. Biotechnology in Husbandry
37. Biotechnology in Environment
38. Biotechnology in Environment
39. Biotechnology in Environment
42. Biotechnology in Indonesia ?
43. Report on Adoption of Plant Biotech
44. Report on Adoption of Plant Biotech