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School of Pharmacy:. School of Pharmacy:. Analysis of Coenzyme Q 10. By Taylor Sineath 6-1-01. Purpose. To test the qualities of Coenzyme Q10 dietary supplements using analytical methods. What is Coenzyme Q 10?.

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analysis of coenzyme q 10

School of Pharmacy:

School of Pharmacy:

Analysis of Coenzyme Q 10

By Taylor Sineath

6-1-01

purpose
Purpose

To test the qualities of Coenzyme Q10 dietary supplements using analytical methods.

what is coenzyme q 10
What is Coenzyme Q 10?

Naturally occurring compound found in organisms. Used for a variety of indications notably cardiovascular diseases.

forms dosage
Forms/Dosage
  • GNC/FoodLion both 10 mg capsules
  • GNC is in a capsule form
  • FoodLion is in soft gel form
testing methods
Testing Methods
  • Weight variation
  • Disintegration
  • HPLC Assay
weight variation
Weight variation
  • Tests the variation in weight of capsules
  • Tests the deviation from the average
  • Tests the net fill of soft gel (FoodLion)
  • Tests the weight of the capsules (GNC)
disintegration
Disintegration
  • Tests the solubility in an environment supposed to imitate that of the stomach
  • Used 6 capsules in sodium acetate buffer
  • 37oC +/- 2oC
  • The limit was 45min
hplc assay
HPLC Assay
  • Determine concentration of analyte within a sample
  • Measures it by light absorption
  • Analyzed 3 capsules per lot
hplc setup
HPLC Setup
  • Used a wavelength of 275nm
  • Flow Rate 1ml/min
  • Column 150mm x 4.6mm Altima C18 3
  • Mobile Phase (25:75) Hexanes:Methanol
  • Injection volume of 20 ul
sample preparation soft gels
Sample Preparation – Soft gels
  • Cut open the shell using forceps and a scalpel over a funnel in a 100ml volumetric flask
  • Wash the funnel,forceps and the scalpel into the 100ml flask with 20ml of hexanes
  • Sonicate for 5 min and diluted to volume with methanol
  • Then sonicate again for 5 min
sample prep cont
Sample Prep. (cont.)
  • Pipetted 2ml of the sample into a 50ml volumetric flask and diluted with methanol
  • Filled a HPLC vial 1/2 to 3/4 full
conclusions
Conclusions
  • Weight variations within brands consistent
  • Disintegrations within brands consistent
  • Assay would indicate variability within the lot and from lot to lot
  • Preliminary data suggests that FoodLion would be a better choice
    • Costs less $10 vs. $17
    • 100 capsules vs. 90 capsule in bottle
    • Less variability
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