Analysis of coenzyme q 10
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Analysis of Coenzyme Q 10 PowerPoint PPT Presentation


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School of Pharmacy:. School of Pharmacy:. Analysis of Coenzyme Q 10. By Taylor Sineath 6-1-01. Purpose. To test the qualities of Coenzyme Q10 dietary supplements using analytical methods. What is Coenzyme Q 10?.

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Analysis of Coenzyme Q 10

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School of Pharmacy:

School of Pharmacy:

Analysis of Coenzyme Q 10

By Taylor Sineath

6-1-01


Purpose

To test the qualities of Coenzyme Q10 dietary supplements using analytical methods.


What is Coenzyme Q 10?

Naturally occurring compound found in organisms. Used for a variety of indications notably cardiovascular diseases.


Forms/Dosage

  • GNC/FoodLion both 10 mg capsules

  • GNC is in a capsule form

  • FoodLion is in soft gel form


Testing Methods

  • Weight variation

  • Disintegration

  • HPLC Assay


Weight variation

  • Tests the variation in weight of capsules

  • Tests the deviation from the average

  • Tests the net fill of soft gel (FoodLion)

  • Tests the weight of the capsules (GNC)


Disintegration

  • Tests the solubility in an environment supposed to imitate that of the stomach

  • Used 6 capsules in sodium acetate buffer

  • 37oC +/- 2oC

  • The limit was 45min


HPLC Assay

  • Determine concentration of analyte within a sample

  • Measures it by light absorption

  • Analyzed 3 capsules per lot


HPLC Setup

  • Used a wavelength of 275nm

  • Flow Rate 1ml/min

  • Column 150mm x 4.6mm Altima C18 3

  • Mobile Phase (25:75) Hexanes:Methanol

  • Injection volume of 20 ul


Sample Chromatogram


Sample Preparation – Soft gels

  • Cut open the shell using forceps and a scalpel over a funnel in a 100ml volumetric flask

  • Wash the funnel,forceps and the scalpel into the 100ml flask with 20ml of hexanes

  • Sonicate for 5 min and diluted to volume with methanol

  • Then sonicate again for 5 min


Sample Prep. (cont.)

  • Pipetted 2ml of the sample into a 50ml volumetric flask and diluted with methanol

  • Filled a HPLC vial 1/2 to 3/4 full


Data from FoodLion Weight Variation


Data From GNC Weight Variation


Disintegration of FoodLion Brand


Disintegration of GNC Brand


Assay of FoodLion Soft gels


Assay of GNC capsules


Conclusions

  • Weight variations within brands consistent

  • Disintegrations within brands consistent

  • Assay would indicate variability within the lot and from lot to lot

  • Preliminary data suggests that FoodLion would be a better choice

    • Costs less $10 vs. $17

    • 100 capsules vs. 90 capsule in bottle

    • Less variability


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