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Our Operational Taxonomic Unit (OTU)-based prokaryotic diversity analysis platform aims to characterize prokaryotic diversity productively and economically by utilizing the advanced next/third generation sequencing technology and other molecular biology methods. Our experienced experts employ this platform to assist our clients in meeting both scientific and regulatory requirements.<br>
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Prokaryotic Diversity Analysis Platform Our Operational Taxonomic Unit (OTU)-based prokaryotic diversity analysis platform aims to characterize prokaryotic diversity productively and economically by utilizing the advanced next/third generation sequencing technology and other molecular biology methods. Our experienced experts employ this platform to assist our clients in meeting both scientific and regulatory requirements. Our Advantages: Cloud-based platform for bioinformatics analysis. • Extensive experience. • Targeted region sequencing, enabling a rapid and efficient workflow. • More than 70% of our team is PhD and Masters-level scientists. • Advanced sequencing platforms. • Tell Us About Your Project We are dedicated to providing outstanding customer service, listening to customer requests and being reachable at all times. REQUEST A QUOTE Introduction to Our Prokaryotic Diversity Analysis Platform
Our integrated prokaryotic diversity analysis platform can be used for profiling of prokaryotic community in your samples utilizing diverse molecular biology techniques. The principle of our prokaryotic diversity detection platform is based on the role of 16S rRNA as the molecular marker in prokaryotes. 16S rRNA gene has nine conserved regions and nine hypervariable regions. Among them, V4-V5 region is the best choice for bacterial diversity analysis and annotation due to its high specificity and large amount of database information. The two sets of primers (519F/915R and 344F/915R) offer the highest resolution for archaebacterial diversity analysis. This platform enables both short-read sequencing by Illumina HiSeq/Roche 454 instruments and full-length 16S rRNA sequencing by PacBio SMRT technology. The generated sequencing data are validated by Sanger sequencing and q-PCR with genus- and species-specific primers. qPCR can also be used to quantify microbial species. Based on this platform, we provide bacterial and archaebacteria diversity analysis. Bacteria and archaea are found in a broad range of habitats, such as soils, water, marshlands, and human body. Archaea even survive in harsh environments. The insight into prokaryotic community structure is an efficient way to understand the role of prokaryotes played in environments or human body, hence making use of prokaryotes or their products. Therefore, prokaryotic diversity analysis has been applied in a variety of fields, including basic research (such as prokaryotic evolution and epidemiologic studies), agricultural practice, environmental protection, as well as pharmaceutical, food, healthcare, and other industries. Additionally, we also have eukaryotic diversity analysis platform for eukaryotic samples (such as fungi, algae, protozoa).
Prokaryotic Diversity Analysis Workflow Bioinformatics Analysis Our bioinformatics analysis includes species annotation, diversity analysis, evolutionary analysis, functional profiling, and correlation analysis. The detailed bioinformatics analysis package is outlined in the following table. Table 1. Our bioinformatics analysis package for prokaryotic diversity detection. Sample Requirement
• 1.8 < OD260/280 < 2.0, 1.8 < OD260/280 < 2.0, no degradation or slight degradation. Illumina platform: DNA amount ≥ 300 ng, PCR Products ≥400 ng PacBio platform: gDNA ≥100 ng, PCR Products ≥400 ng Deliverables: raw sequencing data (FASTQ), trimmed and stitched sequences (FASTA), quality-control dashboard, q-PCR data, statistic data, and your designated bioinformatics report. Original https://www.cd-genomics.com/microbioseq/prokaryotic-diversity-analysis-platform.html Source:
